Liu Jianlong, Deng Zhiwei, Liang Chenglin, Sun Hongwei, Li Dingli, Song Jiankun, Zhang Shaoling, Wang Ran
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
Haidu College, Qingdao Agricultural University, Laiyang 265200, China.
Int J Mol Sci. 2021 May 25;22(11):5567. doi: 10.3390/ijms22115567.
Related to ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 (ABI3/VP1, RAV), transcription factors (TFs) belonging to the APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) TF family play critical roles in plant growth, development, and responses to abiotic and biotic stress. In this study, 11 novel RAV TFs were identified in pear ( Rehd). A phylogenetic analysis revealed that the TFs clustered into three groups with 10 conserved motifs, some of which were group- or subgroup-specific, implying that they are important for the functions of the RAVs in these clades. RAVs in and were closely related, and the former showed a collinear relationship. Analysis of their expression patterns in different tissues and at various growth stages and their responses to abiotic and biotic stress suggested that and play important roles in drought stress and salt stress, respectively. We investigated the function of RAVs in pear peel coloration using two red pear varieties with different color patterns and applying data from transcriptome analyses. We found that participates in the regulation of pericarp color. These findings provide insight into a new TF family in pear and a basis for further studies on the response to drought stress and fruit coloration in this commercially important crop.
与脱落酸不敏感3/胎萌1（ABI3/VP1，RAV）相关，属于APETALA2/乙烯响应因子（AP2/ERF）转录因子家族的转录因子（TFs）在植物生长、发育以及对非生物和生物胁迫的响应中发挥着关键作用。在本研究中，在梨（Rehd）中鉴定出11个新的RAV转录因子。系统发育分析表明，这些转录因子聚为三组，具有10个保守基序，其中一些是组或亚组特异性的，这意味着它们对这些进化枝中RAV的功能很重要。和中的RAV密切相关，且前者呈现共线关系。对它们在不同组织和不同生长阶段的表达模式以及对非生物和生物胁迫的响应分析表明，和分别在干旱胁迫和盐胁迫中发挥重要作用。我们利用两个具有不同颜色模式的红梨品种并结合转录组分析数据，研究了RAV在梨果皮着色中的功能。我们发现参与了果皮颜色的调控。这些发现为梨中一个新的转录因子家族提供了见解，并为进一步研究这种商业上重要作物对干旱胁迫的响应和果实着色奠定了基础。
