Department of Social Medicine and Public Health, Medical University of Warsaw, ul. Oczki 3, 02-007 Warsaw, Poland.
Laboratory of Combating Infectious Agents and Biological Contaminations, Department of Bacteriology and Biocontamination Control, National Institute of Public Health - National Institute of Hygiene, ul. Chocimska 24; 00-791 Warsaw, Poland.
Int J Environ Res Public Health. 2019 Sep 18;16(18):3475. doi: 10.3390/ijerph16183475.
The effectiveness of ready-to-use disinfectant wipes was previously assessed in standardized suspension tests, which were inadequate because they ignored that the wipes are rubbed against a surface. Thus, we assessed the effectiveness of commercially available disinfectant wipes impregnated with an alcoholic solution according to the 16615 standard, which includes a test with mechanical action.
According to the EN 16615 standard, under clean conditions, four squares (5cm x 5 cm), placed next to one another, were marked on a test surface. , and were inoculated on the leftmost square, and a wipe impregnated with an alcoholic solution was placed to the left of that square. Then, the wipe was pressed with a 2.5 kg weight and moved to the right and back to the left. After contact times of 1, 5, 10, or 15 minutes, we measured the reduction in bacterial load.
Alcohol-based ready-to-use commercial wipes did not show sufficient bactericidal activity at the contact times of 1, 5, 10 and 15 minutes. Wipes containing propan-1-ol and a mixture of propan-1-ol and propan-2-ol were active against at the contact times of 1 minute and 15 minutes. None of the examined wipes were active against or .
Bactericidal parameters of ready-to-use disinfectant wipes should be determined in surface tests, in addition to suspension tests, because suspension tests do not simulate the conditions under which disinfectant wipes are used in practice.
此前，即用型消毒剂擦拭布的有效性已在标准化悬浮试验中进行了评估，但这些试验并不充分，因为它们忽略了擦拭布与表面的摩擦。因此，我们根据包含机械作用测试的 16615 标准评估了浸渍有酒精溶液的市售消毒剂擦拭布的有效性。
根据 EN 16615 标准，在清洁条件下，在测试表面上标记四个相邻的 5cm x 5cm 正方形。在最左边的正方形上接种 和 ，并在其左侧放置浸渍有酒精溶液的擦拭布。然后，用 2.5 公斤的重量将擦拭布压下，并向左和向右移动。在 1、5、10 或 15 分钟的接触时间后，我们测量细菌负荷的减少量。
在 1、5、10 和 15 分钟的接触时间内，基于酒精的即用型商用擦拭布没有显示出足够的杀菌活性。含有丙-1-醇和丙-1-醇和丙-2-醇混合物的擦拭布在 1 分钟和 15 分钟的接触时间内对 有效。检查的擦拭布均对 或 无效。
除悬浮试验外，还应在表面试验中确定即用型消毒剂擦拭布的杀菌参数，因为悬浮试验不能模拟消毒剂擦拭布在实际使用中的条件。
