Department of Precision Medicine, University of Campania Luigi Vanvitelli, Via L. De Crecchio 7, 80138 Naples, Italy.
Department of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, Italy.
Int J Mol Sci. 2021 May 26;22(11):5677. doi: 10.3390/ijms22115677.
Dietary phenolic compounds possess potent bioactivity against inflammatory pathways of chronic inflammatory conditions, such as type 2 diabetes. Here, the phenolic profile and bioactivity of Italian red wines Gaglioppo, Magliocco, and Nerello Mascalese were characterized. NMR, HPLC/UV-Vis and spectrophotometric characterization showed that Magliocco was the richest wine in monomeric anthocyanins (two-fold), catechins, and low molecular weight phenolics (LMWP). A positive correlation was observed between the polyphenolic content and antioxidant capacity ( < 0.05), with Magliocco displaying the highest antioxidant capacity ( < 0.01). In vitro evidence on the endothelial cell models of insulin resistance and hyperglycemia showed the ability of Magliocco to reduce reactive oxygen species (ROS) ( < 0.01) and cytokine release ( < 0.01) and to upregulate SIRT1 and SIRT6 ( < 0.01). On the whole, the results indicated that the quantitative and qualitative phenolic profiles of red wines influence their in vitro beneficial effects on oxidative and proinflammatory milieu in endothelial cells, showing a positive modulation of SIRT1 and SIRT6, both implied in vascular aging.
膳食酚类化合物对 2 型糖尿病等慢性炎症性疾病的炎症途径具有强大的生物活性。在这里，我们对意大利红葡萄酒加格罗波（Gaglioppo）、马格拉乔（Magliocco）和内罗洛·马萨莱西（Nerello Mascalese）的酚类成分和生物活性进行了表征。NMR、HPLC/UV-Vis 和分光光度法分析表明，马格拉乔葡萄酒中单宁酸、儿茶素和低分子量酚（LMWP）的含量最高（两倍）。多酚含量与抗氧化能力呈正相关（<0.05），其中马格拉乔葡萄酒的抗氧化能力最强（<0.01）。在胰岛素抵抗和高血糖的内皮细胞模型中的体外证据表明，马格拉乔葡萄酒具有降低活性氧（ROS）（<0.01）和细胞因子释放（<0.01）的能力，并能上调 SIRT1 和 SIRT6（<0.01）。总的来说，这些结果表明，红葡萄酒的定量和定性酚类成分会影响其对内皮细胞氧化和促炎环境的体外有益作用，对 SIRT1 和 SIRT6 具有积极的调节作用，SIRT1 和 SIRT6 均与血管老化有关。
