Vico L, Chappard D, Palle S, Bakulin A V, Novikov V E, Alexandre C
Laboratoire de Biologie du Tissu Osseux, Faculté de Médecine, Saint Etienne, France.
Am J Physiol. 1988 Aug;255(2 Pt 2):R243-7. doi: 10.1152/ajpregu.1988.255.2.R243.
Seven male rats were exposed to 7 days of weightlessness in the Soviet mission COSMOS 1667 and compared with seven control rats by bone histomorphometric methods. In proximal tibial metaphysis, the trabecular bone volume was markedly reduced in flight animals. Trabeculae were decreased in number and thickness; this probably leads to alteration of bone mechanical properties. Formation activity (reflected by measurements of osteoid seams) was decreased at trabecular and endosteal levels. Resorption activity (estimated by count of osteoclast number and active resorption surfaces using a histoenzymologic method) remained unchanged. The imbalance between these cellular activities appears to be responsible for the loss of trabecular bone mass. In proximal femoral metaphysis, measurements were performed in an area located under the muscular insertions. The trabecular bone volume, despite a slight decrease in flight rats, was not significantly different from that of control rats. Furthermore, osteoclastic and osteoid parameters were unchanged. Differential responses between these two long bones need additional investigations. In thoracic and lumbar vertebrae no detectable change in bone mass and bone resorption parameters was found.
七只雄性大鼠在苏联的“宇宙1667”任务中经历了7天的失重状态，并通过骨组织形态计量学方法与七只对照大鼠进行了比较。在近端胫骨干骺端，飞行大鼠的骨小梁体积明显减少。骨小梁数量和厚度减少；这可能导致骨力学性能改变。在骨小梁和骨内膜水平，形成活性（通过类骨质缝测量反映）降低。吸收活性（使用组织酶学法通过破骨细胞数量计数和活跃吸收表面估计）保持不变。这些细胞活性之间的失衡似乎是骨小梁骨量丢失的原因。在近端股骨干骺端，在肌肉附着下方的区域进行测量。尽管飞行大鼠的骨小梁体积略有下降，但与对照大鼠相比无显著差异。此外，破骨细胞和类骨质参数未改变。这两块长骨之间的差异反应需要进一步研究。在胸椎和腰椎中，未发现骨量和骨吸收参数有可检测到的变化。
