Animal Model Research Group, Korea Institute of Toxicology, Jeongeup, Jeollabuk-do, Republic of Korea.
PLoS One. 2021 Jun 4;16(6):e0252947. doi: 10.1371/journal.pone.0252947. eCollection 2021.
Minipigs have remarkably similar physiology to humans, therefore, they it can be a good animal model for inflammation study. Thus, the conventional (serum chemistry, histopathology) and novel analytic tools [immune cell identification in tissue, cytokine level in peripheral blood mononuclear cells (PBMC) and serum, NF-kB target gene analysis in tissue] were applied to determine inflammation in Chicago Miniature Swine (CMS) minipig. Lipopolysaccharide (LPS)-induced acute systemic inflammation caused liver and kidney damage in serum chemistry and histopathology. Immunohistochemistry (IHC) also showed an increase of immune cell distribution in spleen and lung during inflammation. Moreover, NF-kB-target gene expression was upregulated in lung and kidney in acute inflammation and in heart, liver, and intestine in chronic inflammation. Cytokine mRNA was elevated in PBMC under acute inflammation along with elevated absolute cytokine levels in serum. Overall, LPS-mediated systemic inflammation affects the various organs, and can be detected by IHC of immune cells, gene analysis in PBMC, and measuring the absolute cytokine in serum along with conventional inflammation analytic tools.
小型猪的生理学与人类非常相似，因此它们可以作为炎症研究的良好动物模型。因此，采用了常规（血清化学、组织病理学）和新型分析工具[组织中免疫细胞的鉴定、外周血单个核细胞（PBMC）和血清中的细胞因子水平、组织中 NF-κB 靶基因分析]来确定芝加哥小型猪（CMS）小型猪的炎症情况。脂多糖（LPS）诱导的急性全身炎症导致血清化学和组织病理学中的肝和肾损伤。免疫组织化学（IHC）还显示在炎症期间脾脏和肺部的免疫细胞分布增加。此外，在急性炎症时 NF-κB 靶基因在肺和肾中上调，在慢性炎症时在心脏、肝脏和肠道中上调。在急性炎症时，PBMC 中的细胞因子 mRNA 升高，同时血清中的绝对细胞因子水平升高。总之，LPS 介导的全身炎症会影响各种器官，可以通过免疫细胞的 IHC、PBMC 中的基因分析以及测量血清中的绝对细胞因子以及常规炎症分析工具来检测。
