Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688, Kraków, Poland.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688, Kraków, Poland.
Chem Biodivers. 2021 Jul;18(7):e2100335. doi: 10.1002/cbdv.202100335. Epub 2021 Jun 4.
Ardisia crenata Sims (Primulaceae) occurs in natural habitats in two varieties, bearing red or white fruits. While roots of the red-berried ardisia are valued as a medicinal product, the pharmacological activity of which is attributed to triterpene saponins, including ardisiacrispin A, data on the white-berried variety are scarce. A TLC-densitometric method was developed and validated to estimate the levels of saponins, calculated as ardisiacrispin A, in different plant parts in both varieties. Their content amounted to 22.17±4.75 and 25.72±1.46 mg/g d.w. in roots, and 2.64±0.74 and 3.43±0.70 mg/g d.w. in fruits of red-berried and white-berried ardisia, respectively. Assessment of cytotoxicity of ardisiacrispin A and A. crenata extracts on a panel of human cancer cell lines revealed a similar effect of root extracts from both varieties, with the highest potency against melanoma WM793 and colon cancer Caco2. Thus, roots of the white-berried variety may be treated as a substitute for red-berried ardisia and serve as an alternative source for the acquisition of plant material rich in bioactive saponins.
紫金牛（紫金牛科）在自然生境中存在两个变种，分别结红色或白色果实。红果紫金牛的根被用作药用，其药理活性归因于三萜皂苷，包括紫金牛苷 A，而关于白果变种的数据则较少。开发并验证了一种 TLC-密度测定法，以估算两种变种中不同植物部位的皂苷（以紫金牛苷 A 计）水平。其含量在红果和白果的根中分别为 22.17±4.75 和 25.72±1.46mg/g dw，在红果和白果的果实中分别为 2.64±0.74 和 3.43±0.70mg/g dw。评估紫金牛苷 A 和紫金牛提取物对一系列人类癌细胞系的细胞毒性，结果表明两种变种的根提取物具有相似的作用，对黑色素瘤 WM793 和结肠癌 Caco2 的活性最强。因此，白果变种的根可以被视为红果紫金牛的替代品，并且是获得富含生物活性皂苷的植物材料的替代来源。
