Cytotoxic 13,28 Epoxy Bridged Oleanane-Type Triterpenoid Saponins from the Roots of .

Ardisiacrispin D-F (-), three new 13,28 epoxy bridged oleanane-type triterpenoid saponins, together with four known analogues (-) were isolated from the roots of . The structures of - were elucidated based on 1D and 2D-NMR experiments and by comparing their spectroscopic data with values from the published literatures. Ardisiacrispin D-F (-) are first examples that the monosaccharide directly linked to aglycone C-3 of triterpenoid saponins in genus are non-arabinopyranose. In the present paper, all compounds are evaluated for the cytotoxicity against three cancer cell lines (HeLa, HepG2 and U87 MG) in vitro. The results show that compounds , and exhibited significant cytotoxicity against Hela and U87 MG cells with IC values in the range of 2.2 ± 0.6 to 9.5 ± 1.8 µM. The present investigation suggests that roots of could be a potential source of natural anti-tumor agents and their triterpenoid saponins might be responsible for cytotoxicity.