Erdogan Hilal, Yildirim Sibel, Cobankara Funda Kont
Department of Endodontics, Faculty of Dentistry, Nevsehir Haci Bektas Veli University, Nevsehir, Turkey.
Department of Pediatric Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkey.
Aust Endod J. 2021 Dec;47(3):645-653. doi: 10.1111/aej.12537. Epub 2021 Jun 7.
The aim of this study was to evaluate the biocompatibility of epoxy-resin-based AHPlus, salicylate-based MTA-Fillapex and calcium silicate-based iRootSP root canal sealers. Cytotoxicity was assessed by XTT test. The extracts from sealers of different setting times were serially diluted. Cell viability was calculated as the percentage of the control group (100%). The optimal concentration of each sealer was used at genotoxicity test, and micronuclei formations were detected. Statistical analyses were done by using Kruskal-Wallis and Dunn post hoc test with Bonferroni correction. AHPlus and MTA-Fillapex showed the lowest percentage of cell viability at higher concentrations (1:1, 1:2, 1:4), especially at first 12 h. iRootSP showed higher viability at all concentrations and times than AHPlus and MTA-Fillapex. At genotoxicity assay, AHPlus increased the number of micronuclei. MTA-Fillapex slightly induced micronucleus formation (not significant) and iRootSP was not increased. In conclusion, calcium silicate-based iRootSP had lowest cytotoxic and genotoxic potential and can be considered as a highly biocompatible material.
本研究的目的是评估基于环氧树脂的AHPlus、基于水杨酸盐的MTA-Fillapex和基于硅酸钙的iRootSP根管封闭剂的生物相容性。通过XTT试验评估细胞毒性。对不同凝固时间的封闭剂提取物进行系列稀释。细胞活力以对照组（100%）的百分比计算。每种封闭剂的最佳浓度用于遗传毒性试验，并检测微核形成情况。采用Kruskal-Wallis检验和Dunn事后检验并进行Bonferroni校正进行统计分析。AHPlus和MTA-Fillapex在较高浓度（1:1、1:2、1:4）下细胞活力百分比最低，尤其是在最初12小时。iRootSP在所有浓度和时间下的活力均高于AHPlus和MTA-Fillapex。在遗传毒性试验中，AHPlus增加了微核数量。MTA-Fillapex轻微诱导微核形成（无显著性），而iRootSP未增加。总之，基于硅酸钙的iRootSP具有最低的细胞毒性和遗传毒性潜力，可被视为一种高度生物相容性材料。
