体外培养菌株提取物的抗增殖和抗菌活性。
Antiproliferative and antibacterial activity of extracts of strains grown in vitro.
作者信息
Serrano-Márquez Leonardo, Trigos Ángel, Couttolenc Alan, Padrón José M, Shnyreva Alla V, Mendoza Guillermo
机构信息
Doctorado en Ciencias Biomédicas, Universidad Veracruzana, Av. Luis Castelazo Ayala s/n, Col. Industrial Animas, 91190 Xalapa, Veracruz, Mexico.
Centro de Investigación en Micología Aplicada, Universidad Veracruzana, Calle Médicos 5, Col. Unidad del Bosque, 91010 Xalapa, Veracruz, Mexico.
出版信息
Food Sci Biotechnol. 2021 May 5;30(5):711-721. doi: 10.1007/s10068-021-00903-1. eCollection 2021 May.
In this bioprospecting study the biological activities of extracts of the in vitro culture of Mexican strains were evaluated. The extracts were tested by the Sulforhodamine B staining method for antiproliferative activity and the plate microdilution method for antibacterial activity. Extracts that proved bioactive in these two activities, the antioxidant activity (Galvinoxyl, ABTS, and DPPH) and total phenolic contents (Folin-Ciocalteu) were additionally determined, as well as acute toxicity (). In the antiproliferative activity strain (GH-16-015) obtained a remarkable value of GI ≤ 50 µg/mL against tumor lines: A549, HBL-100, HeLa, and T-47D. . strains (GH-16-012 and GH-16-015) showed MIC values = 500 µg/mL against . . strain (GH-16-012) almost reduced by 50% the radical Galvinoxyl. Finally, strain (GH-16-023) presented the lowest level of toxicity with a LC of 490.881 µg/mL against . . These results support the potential medicinal effects of Mexican strains of . .
在这项生物勘探研究中，对墨西哥菌株体外培养提取物的生物活性进行了评估。提取物通过磺酰罗丹明B染色法测试其抗增殖活性，并通过平板微量稀释法测试其抗菌活性。对在这两种活性中被证明具有生物活性的提取物，还测定了抗氧化活性（加尔文氧基、ABTS和DPPH）、总酚含量（福林-西奥尔特）以及急性毒性（）。在抗增殖活性方面，菌株（GH-16-015）对肿瘤细胞系A549、HBL-100、HeLa和T-47D的GI值≤50μg/mL，表现出显著活性。。菌株（GH-16-012和GH-16-015）对表现出MIC值=500μg/mL。。菌株（GH-16-012）几乎使加尔文氧基自由基减少了50%。最后，菌株（GH-16-023）对。。表现出最低的毒性水平，LC为490.881μg/mL。这些结果支持了墨西哥菌株的潜在药用效果。。
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