Henrotin Yves, Antoine Christophe, Zwerts Elodie, Neutelings Thibaut, Bouvret Elodie
Artialis SA, Tour GIGA CHU Sart-Tilman Liège Belgium.
Bone and Cartilage Research Unit Center for Interdisciplinary Research on Medicines, Arthropôle Liège Institute of Pathology University of Liège, CHU Sart-Tilman Liège Belgium.
Food Sci Nutr. 2021 Apr 10;9(6):2877-2885. doi: 10.1002/fsn3.2244. eCollection 2021 Jun.
The objective of this study was to evaluate the effects of oral fish cartilage hydrolysate (FCH) on symptoms and joint tissue structure in rat developing osteoarthritis induced surgically. Osteoarthritis was induced in the right knee of mature male Lewis rats ( = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Two weeks after surgery, rats were treated orally with either control (sterile HO) or FCH for four weeks. Pain and function were assessed by dynamic weight-bearing test (incapacitance test), electronic Von Frey (EVF; hindpaw allodynia threshold), and pressure algometer (knee allodynia threshold). Time and groups differences at each time point were evaluated using a mixed model. The histological features were evaluated eight weeks after surgery using OARSI score. Mann-Whitney test nonparametric test was applied to compare OARSI score. ACTL/pMMx surgery significantly reduced weight-bearing and increased allodynia and sensitivity thresholds of the operated paw/knee. Globally, FCH improved these parameters faster, but no significant difference between control and FCH groups was observed. Eight weeks after surgery, rats developed moderate OA lesions. Compared with control, FCH did not significantly modify OA lesion severity assessed using the OARSI score. In this mechanically induced OA model, 4 weeks of supplementation with FCH had no significant effect on cartilage lesion, but tends to accelerate pain relief and joint function recovery. This positive trend may have opened the way for further investigation of FCH as potential treatment of joint discomfort associated with OA.
本研究的目的是评估口服鱼软骨水解物（FCH）对手术诱导的大鼠发育性骨关节炎症状和关节组织结构的影响。通过手术切断前交叉韧带（ACLT）并结合部分内侧半月板切除术（pMMx），在成熟雄性Lewis大鼠（每组n = 12）的右膝诱导骨关节炎。手术后两周，大鼠口服给予对照物（无菌水）或FCH，持续四周。通过动态负重试验（失能试验）、电子von Frey（EVF；后爪痛觉过敏阈值）和压力痛觉计（膝关节痛觉过敏阈值）评估疼痛和功能。使用混合模型评估每个时间点的时间和组间差异。术后八周使用OARSI评分评估组织学特征。应用Mann-Whitney检验非参数检验比较OARSI评分。ACLT/pMMx手术显著降低了负重，并增加了手术爪/膝关节的痛觉过敏和感觉阈值。总体而言，FCH能更快地改善这些参数，但对照组和FCH组之间未观察到显著差异。术后八周，大鼠出现中度OA病变。与对照组相比，FCH并未显著改变使用OARSI评分评估的OA病变严重程度。在这个机械诱导的OA模型中，补充FCH 4周对软骨病变没有显著影响，但倾向于加速疼痛缓解和关节功能恢复。这种积极趋势可能为进一步研究FCH作为OA相关关节不适潜在治疗方法开辟了道路。
