Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.
Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA.
Nat Commun. 2021 Jun 18;12(1):3764. doi: 10.1038/s41467-021-24104-w.
Post-surgical cardiac adhesions represent a significant problem during routine cardiothoracic procedures. This fibrous tissue can impair heart function and inhibit surgical access in reoperation procedures. Here, we propose a hydrogel barrier composed of oxime crosslinked poly(ethylene glycol) (PEG) with the inclusion of a catechol (Cat) group to improve retention on the heart for pericardial adhesion prevention. This three component system is comprised of aldehyde (Ald), aminooxy (AO), and Cat functionalized PEG mixed to form the final gel (Ald-AO-Cat). Ald-AO-Cat has favorable mechanical properties, degradation kinetics, and minimal swelling, as well as superior tissue retention compared to an initial Ald-AO gel formulation. We show that the material is cytocompatible, resists cell adhesion, and led to a reduction in the severity of adhesions in an in vivo rat model. We further show feasibility in a pilot porcine study. The Ald-AO-Cat hydrogel barrier may therefore serve as a promising solution for preventing post-surgical cardiac adhesions.
术后心脏粘连是心脏胸外科手术中一个严重的问题。这种纤维组织会损害心脏功能，并影响再次手术时的手术操作。在这里，我们提出了一种水凝胶屏障，由肟交联的聚乙二醇（PEG）组成，其中包含一个儿茶酚（Cat）基团，以改善在心包粘连预防中的保留。该三组分系统由醛（Ald）、氨氧基（AO）和 Cat 功能化 PEG 混合形成最终凝胶（Ald-AO-Cat）。与初始的 Ald-AO 凝胶配方相比，Ald-AO-Cat 具有良好的机械性能、降解动力学和最小的肿胀，以及更好的组织保留。我们证明了该材料具有细胞相容性，能抵抗细胞黏附，并在体内大鼠模型中减少粘连的严重程度。我们还在初步的猪研究中证明了其可行性。因此，Ald-AO-Cat 水凝胶屏障可能是一种有前途的预防术后心脏粘连的解决方案。
Zotero 插件
