Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
Int J Syst Evol Microbiol. 2021 Jun;71(6). doi: 10.1099/ijsem.0.004808.
Two Gram-stain-negative, moderately halophilic, non-motile, rod-shaped, pale yellow, and aerobic strains, designated WDS1C4 and WDS4C29, were isolated from a marine solar saltern in Weihai, Shandong Province, PR China. Growth of strain WDS1C4 occurred at 10-45 °C (optimum, 37 °C), with 4-16 % (w/v) NaCl (optimum, 8 %) and at pH 6.5-9.0 (optimum, pH 7.5). Growth of strain WDS4C29 occurred at 10-45 °C (optimum, 40 °C), with 2-18 % (w/v) NaCl (optimum, 6 %) and at pH 6.5-9.0 (optimum, pH 7.5). Q-10 was the sole respiratory quinone of the two strains. The major polar lipids of strains WDS1C4 and WDS4C29 were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major cellular fatty acid in strains WDS1C4 and WDS4C29 was C 7, and the genomic DNA G+C contents of strains WDS1C4 and WDS4C29 were 67.6 and 63.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains WDS1C4 and WDS4C29 were members of the family and showed 94.3 and 95.3 % similarities to their closest relative, , respectively. The similarity between WDS1C4 and WDS4C29 was 97.3 %. Differential phenotypic and genotypic characteristics of the two isolates from recognized genera showed that the two strains should be classified as representing two novel species in a new genus for which the names gen. nov., sp. nov. (type species, type strain WDS1C4=MCCC 1H00179=KCTC 52542) and sp. nov. (WDS4C29=MCCC 1H00175=KCTC 52541) are proposed.
两株革兰氏阴性、中度嗜盐、不运动、杆状、浅黄色、需氧的菌株，分别命名为 WDS1C4 和 WDS4C29，从中国山东省威海市的一个海洋太阳盐场中分离得到。WDS1C4 菌株的生长温度为 10-45°C（最佳温度为 37°C），可利用 4-16%（w/v）NaCl（最佳浓度为 8%），pH 值为 6.5-9.0（最佳 pH 值为 7.5）。WDS4C29 菌株的生长温度为 10-45°C（最佳温度为 40°C），可利用 2-18%（w/v）NaCl（最佳浓度为 6%），pH 值为 6.5-9.0（最佳 pH 值为 7.5）。Q-10 是这两株菌的唯一呼吸醌。菌株 WDS1C4 和 WDS4C29 的主要极性脂是磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。菌株 WDS1C4 和 WDS4C29 的主要细胞脂肪酸是 C7，菌株 WDS1C4 和 WDS4C29 的基因组 DNA G+C 含量分别为 67.6 和 63.3 mol%。基于 16S rRNA 基因序列的系统发育分析表明，WDS1C4 和 WDS4C29 菌株是 科的成员，与最接近的亲缘种 相似度分别为 94.3%和 95.3%。WDS1C4 和 WDS4C29 之间的相似度为 97.3%。这两个分离株与公认属的菌株在表型和基因型上的差异表明，这两个菌株应被归类为代表一个新属的两个新种，该新属命名为 ，新种命名为 （模式种，模式菌株 WDS1C4=MCCC 1H00179=KCTC 52542）和 （WDS4C29=MCCC 1H00175=KCTC 52541）。
