Albano Mariana, Greenwood-Quaintance Kerryl E, Karau Melissa J, Mandrekar Jayawant N, Patel Robin
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.
Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, United States.
Int J Cardiol Heart Vasc. 2021 Jun 8;34:100801. doi: 10.1016/j.ijcha.2021.100801. eCollection 2021 Jun.
Antibiotic envelopes are being developed for cardiac implantable electronic device (CIED) wrapping to reduce the risk of infections.
Fifteen CIED infection-associated bacterial isolates of and were used to assess biofilm formation on Hylomate® compared to titanium, silicone and polyurethane coupons pre-treated with vancomycin (400 µg/ml), bacitracin (1000 U/ml) or a combination of rifampin (80 µg/ml) plus minocycline (50 µg/ml). Scanning electron microscopy (SEM) was performed to visualize bacteria on Hylomate®.
There was significantly less (p < 0.05) and on Hylomate® pre-treated with vancomycin, bacitracin or rifampin plus minocycline after 24 h of incubation (≤1.00 log CFU/cm) compared with titanium, silicone or polyurethane pre-treated with vancomycin, bacitracin or rifampin plus minocycline. . biofilms were not detected (≤1.00 log CFU/cm) on pre-treated Hylomate® coupons.
This study showed that Hylomate® coupons pre-treated with antibiotics reduced staphylococcal and biofilm formation
正在研发用于包裹心脏植入式电子设备（CIED）的抗生素包膜，以降低感染风险。
使用15株与CIED感染相关的金黄色葡萄球菌和表皮葡萄球菌分离株，与用万古霉素（400μg/ml）、杆菌肽（1000 U/ml）或利福平（80μg/ml）加米诺环素（50μg/ml）预处理的钛、硅胶和聚氨酯试片相比，评估其在Hylomate®上的生物膜形成情况。进行扫描电子显微镜（SEM）观察Hylomate®上的细菌。
与用万古霉素、杆菌肽或利福平加米诺环素预处理的钛、硅胶或聚氨酯相比，在孵育24小时后，用万古霉素、杆菌肽或利福平加米诺环素预处理的Hylomate®上的金黄色葡萄球菌和表皮葡萄球菌显著减少（p<0.05）（≤1.00 log CFU/cm）。在预处理的Hylomate®试片上未检测到表皮葡萄球菌生物膜（≤1.00 log CFU/cm）。
本研究表明，用抗生素预处理的Hylomate®试片可减少葡萄球菌和表皮葡萄球菌生物膜的形成
