Endo E G, Yao X Y, Marmor M F
Department of Ophthalmology, Stanford University Medical Center, CA 94305.
Invest Ophthalmol Vis Sci. 1988 Sep;29(9):1390-6.
Retinal adhesion deteriorates rapidly after enucleation. We have developed a new in vitro method for measuring retinal adhesiveness in the rabbit that is faster than previous models, and can monitor changes sooner after enucleation. We used the percentage of retina which retained pigment after peeling from the RPE as a quantitative measure of retinal adhesiveness. We found that the failure of adhesion after death is more rapid and severe than previously reported, but can be inhibited by cold temperature. Pigment adherence was also modified by ionic and metabolic factors that have been found to affect other indices of adhesion. These results emphasize the limitations of in vitro data relative to the physiologic forces that maintain retinal adhesion in vivo.
眼球摘除术后视网膜粘连迅速恶化。我们开发了一种新的体外方法来测量兔子的视网膜粘连性，该方法比以前的模型更快，并且能够在眼球摘除后更快地监测变化。我们将从视网膜色素上皮（RPE）剥离后仍保留色素的视网膜百分比用作视网膜粘连性的定量指标。我们发现，死后粘连失败比先前报道的更快且更严重，但可被低温抑制。色素附着也受到离子和代谢因素的影响，这些因素已被发现会影响其他粘连指标。这些结果强调了体外数据相对于体内维持视网膜粘连的生理力的局限性。
