Jaradat Nidal, Dacca Hanaa, Hawash Mohammed, Abualhasan Murad N
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, 00970, Nablus, State of Palestine.
BMC Chem. 2021 Jun 26;15(1):41. doi: 10.1186/s13065-021-00768-9.
Discovering and screening for potential anti-obesity, anti-diabetic, anticancer, and antioxidant treatments from natural products still in recent times the main goal for many pharmaceutical scientists. The present investigation aimed to evaluate the chemical constituents of Ephedra alata fruits various extracts and to assess their antioxidant, antiobesity, antidiabetic, and cytotoxic effects.
In this work, high content of flavonoids and phenols were observed in the methanol fraction of E. alata fruits, which reached 98.95 mg of RUE/g and 33.22 mg of GAE/g, respectively. The methanol fraction has significant inhibitory activity against DPPH, α-amylase, α-glucosidase, and lipase with an IC value of 1.07, 9.43, 46.16, and 66.48 µg/mL. respectively. Also has anticancer activity against HeLa cancer cell line. While the acetone fraction has potent antioxidant activity with IC 5 µg/mL.
The DPPH and digestive enzymes assays results showed that the methanolic fraction of E. alata fruits has potent antioxidant, anti-diabetic, and anti-obesity activities, which can be an excellent candidate for biological and chemical analysis and can be further subjected for isolation of the therapeutically active compounds with anticancer potency.
从天然产物中发现并筛选潜在的抗肥胖、抗糖尿病、抗癌和抗氧化治疗药物仍是许多药物科学家近期的主要目标。本研究旨在评估翅麻黄果实不同提取物的化学成分，并评估其抗氧化、抗肥胖、抗糖尿病和细胞毒性作用。
在本研究中，翅麻黄果实甲醇提取物中黄酮类化合物和酚类物质含量较高，分别达到98.95毫克芦丁当量/克和33.22毫克没食子酸当量/克。甲醇提取物对DPPH、α-淀粉酶、α-葡萄糖苷酶和脂肪酶具有显著的抑制活性，IC值分别为1.07、9.43、46.16和66.48微克/毫升。对人宫颈癌HeLa细胞系也具有抗癌活性。而丙酮提取物具有较强的抗氧化活性，IC50为5微克/毫升。
DPPH和消化酶检测结果表明，翅麻黄果实甲醇提取物具有较强的抗氧化、抗糖尿病和抗肥胖活性，可作为生物和化学分析的优良候选物，并可进一步用于分离具有抗癌潜力的治疗活性化合物。
