Centre for Crop Health, University of Southern Queensland, Australia.
Department of Agriculture and Fisheries, Queensland, Australia.
Mol Plant Microbe Interact. 2021 Oct;34(10):1216-1222. doi: 10.1094/MPMI-05-21-0116-A. Epub 2021 Oct 28.
Despite the substantial economic impact of pv. on legume production worldwide, the genetic basis of its pathogenicity and potential host association is poorly understood. The production of high-quality reference genome assemblies of pv. strains associated with different hosts sheds light on the genetic basis of its pathogenic variability and host association. Moreover, the study of recent outbreaks of bacterial wilt and microevolution of the pathogen in Australia requires access to high-quality reference genomes that are sufficiently closely related to the population being studied within Australia. We provide the first genome assemblies of pv. strains associated with mungbean and soybean, which revealed high variability in their plasmid composition. The analysis of pv. genomes revealed an extensive suite of carbohydrate-active enzymes potentially associated with pathogenicity, including four carbohydrate esterases, 50 glycoside hydrolases, 23 glycosyl transferases, and a polysaccharide lyase. We also identified 11 serine peptidases, three of which were located within a linear plasmid, pCff119. These high-quality assemblies and annotations will provide a foundation for population genomics studies of pv. in Australia and for answering fundamental questions regarding pathogenicity factors and adaptation of pv. to various hosts worldwide and, at a broader scale, contribute to unraveling genomic features of gram-positive, xylem-inhabiting bacterial pathogens.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
尽管 pv. 对世界范围内豆科植物生产造成了巨大的经济影响，但人们对其致病性和潜在宿主关联性的遗传基础知之甚少。高质量的 pv. 与不同宿主相关菌株的参考基因组组装的产生，揭示了其致病性变异性和宿主关联性的遗传基础。此外，对澳大利亚细菌性萎蔫病的近期暴发和病原体的微进化的研究需要获得与澳大利亚境内研究人群足够接近的高质量参考基因组。我们提供了与绿豆和大豆相关的 pv. 菌株的第一个基因组组装，揭示了它们质粒组成的高度变异性。对 pv. 基因组的分析揭示了一套广泛的碳水化合物活性酶，这些酶可能与致病性有关，包括四个碳水化合物酯酶、50 个糖苷水解酶、23 个糖基转移酶和一个多糖裂解酶。我们还鉴定了 11 种丝氨酸肽酶，其中 3 种位于线性质粒 pCff119 内。这些高质量的组装和注释将为 pv. 在澳大利亚的群体基因组学研究提供基础，并为回答有关致病性因素和 pv. 对世界各地各种宿主的适应性的基本问题提供答案，并且在更广泛的范围内，有助于揭示革兰氏阳性、木质部栖息的细菌病原体的基因组特征。[公式：见正文]版权所有 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
