Ngalimat Mohamad Syazwan, Raja Abd Rahman Raja Noor Zaliha, Yusof Mohd Termizi, Syahir Amir, Sabri Suriana
Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
PeerJ. 2019 Aug 22;7:e7478. doi: 10.7717/peerj.7478. eCollection 2019.
Bacteria are present in stingless bee nest products. However, detailed information on their characteristics is scarce. Thus, this study aims to investigate the characteristics of bacterial species isolated from Malaysian stingless bee, , nest products. Honey, bee bread and propolis were collected aseptically from four geographical localities of Malaysia. Total plate count (TPC), bacterial identification, phenotypic profile and enzymatic and antibacterial activities were studied. The results indicated that the number of TPC varies from one location to another. A total of 41 different bacterial isolates from the phyla Firmicutes, Proteobacteria and Actinobacteria were identified. species were the major bacteria found. Therein, was the most frequently isolated species followed by , , , , , , , , , , , , and . Phenotypic profile of 15 bacterial isolates using GEN III MicroPlate™ system revealed most of the isolates as capable to utilise carbohydrates as well as amino acids and carboxylic acids and derivatives. Proteolytic, lipolytic and cellulolytic activities as determined by enzymatic assays were detected in PD6, PD9, BD3 and BD9. PD9 showed broad-spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria in vitro. The multienzymes and antimicrobial activities exhibited by the bacterial isolates from nest products could provide potential sources of enzymes and antimicrobial compounds for biotechnological applications.
无刺蜂蜂巢产品中存在细菌。然而，关于其特性的详细信息却很匮乏。因此，本研究旨在调查从马来西亚无刺蜂蜂巢产品中分离出的细菌种类的特性。从马来西亚的四个地理区域无菌采集了蜂蜜、蜂粮和蜂胶。研究了总平板计数（TPC）、细菌鉴定、表型特征以及酶活性和抗菌活性。结果表明，TPC的数量因地点而异。共鉴定出41种来自厚壁菌门、变形菌门和放线菌门的不同细菌分离株。 是主要发现的细菌。其中， 是最常分离出的菌株，其次是 、 、 、 、 、 、 、 、 、 、 、 、 和 。使用GEN III MicroPlate™系统对15株细菌分离株进行的表型分析表明，大多数分离株能够利用碳水化合物以及氨基酸和羧酸及其衍生物。通过酶促试验测定，在 PD6、 PD9、 BD3和 BD9中检测到了蛋白水解、脂肪分解和纤维素分解活性。 PD9在体外对革兰氏阳性菌和革兰氏阴性菌均表现出广谱抗菌活性。从无刺蜂蜂巢产品中分离出的细菌所表现出的多种酶活性和抗菌活性可为生物技术应用提供潜在的酶和抗菌化合物来源。
