Lee Min-Kyu, Lee Hyun, Kim Hyoun-Ee, Lee Eun-Jung, Jang Tae-Sik, Jung Hyun-Do
Department of Materials Science and Engineering, Seoul National University, Seoul 08826, Korea.
Department of Materials Science and Engineering and Querrey-Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208, USA.
Nanomaterials (Basel). 2021 Jun 7;11(6):1507. doi: 10.3390/nano11061507.
Nano-scale surface roughening of metallic bio-implants plays an important role in the clinical success of hard tissue reconstruction and replacement. In this study, the nano-topographical features of titanium-niobium-zirconium (TNZ) alloy surfaces were controlled by using the target-ion induced plasma sputtering (TIPS) technique to improve the in vitro osteoblastic response. The TIPS technique is a novel strategy for etching the surface of metallic bio-implants using bombardment of target metal cations, which were accelerated by an extremely high negative bias voltage applied to the substrates. The nano-topography of the TNZ surfaces was successfully controlled by modulating experimental variables (such as the ion etching energy and the type of substrate or target materials) of TIPS. As a result, various nanopatterns (size: 10-210 nm) were fabricated on the surface of the TNZ alloys. Compared with the control group, experimental groups with nanopattern widths of ≥130 nm (130 and 210 nm groups) exhibited superior cell adhesion, proliferation, and differentiation. Our findings demonstrate that TIPS is a promising technology that can impart excellent biological functions to the surface of metallic bio-implants.
金属生物植入物的纳米级表面粗糙化在硬组织重建和置换的临床成功中起着重要作用。在本研究中，通过使用靶离子诱导等离子体溅射（TIPS）技术来控制钛-铌-锆（TNZ）合金表面的纳米拓扑特征，以改善体外成骨细胞反应。TIPS技术是一种利用靶金属阳离子轰击来蚀刻金属生物植入物表面的新策略，这些阳离子由施加在基底上的极高负偏压加速。通过调节TIPS的实验变量（如离子蚀刻能量以及基底或靶材料的类型），成功控制了TNZ表面的纳米拓扑。结果，在TNZ合金表面制备出了各种纳米图案（尺寸：10 - 210 nm）。与对照组相比，纳米图案宽度≥130 nm的实验组（130和210 nm组）表现出更好的细胞粘附、增殖和分化。我们的研究结果表明，TIPS是一项有前景的技术，能够赋予金属生物植入物表面优异的生物学功能。
