从一名免疫功能低下患者的粪便中分离出活的严重急性呼吸综合征冠状病毒2（SARS-CoV-2），提示可能存在粪便传播途径。
Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission.
作者信息
Dergham Julie, Delerce Jeremy, Bedotto Marielle, La Scola Bernard, Moal Valérie
机构信息
IHU-Méditerranée Infection, 13005 Marseille, France.
Institut de Recherche pour le Développement (IRD), Assistance Publique Hôpitaux de Marseille, Microbes Evolution Phylogeny and Infections (MEPHI), Aix Marseille Université, 13005 Marseille, France.
出版信息
J Clin Med. 2021 Jun 18;10(12):2696. doi: 10.3390/jcm10122696.
(1) Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) excretion in stools is well documented by RT-PCR, but evidences that stools contain infectious particles are scarce. (2) Methods: After observing a Corona Virus 2019 Disease (COVID-19) epidemic cluster associated with a ruptured sewage pipe, we search for such a viable SARS-CoV-2 particle in stool by inoculating 106 samples from 46 patients. (3) Results: We successfully obtained two isolates from a unique patient with kidney transplantation under immunosuppressive therapy who was admitted for severe diarrhea. (4) Conclusions: This report emphasizes that SARS-CoV-2 is an enteric virus, and infectious virus particles can be isolated from the stool of immune-compromised patients like, in our case, kidney transplant recipient. Immune-compromised patients are likely to have massive multiplication of the virus in the gastrointestinal tract and this report suggests possible fecal transmission of SARS-CoV-2.
（1）背景：通过逆转录聚合酶链反应（RT-PCR）已充分证明严重急性呼吸综合征冠状病毒2（SARS-CoV-2）可经粪便排出，但粪便中含有传染性颗粒的证据却很少。（2）方法：在观察到一起与污水管破裂相关的2019冠状病毒病（COVID-19）疫情聚集性病例后，我们通过接种46例患者的106份样本，在粪便中寻找这种有活性的SARS-CoV-2颗粒。（3）结果：我们成功从一名接受免疫抑制治疗的肾移植患者（因严重腹泻入院）的粪便中获得了两株病毒分离株。（4）结论：本报告强调SARS-CoV-2是一种肠道病毒，并且可以从免疫功能低下患者（如我们病例中的肾移植受者）的粪便中分离出有传染性的病毒颗粒。免疫功能低下患者的胃肠道中病毒可能大量增殖，本报告提示SARS-CoV-2可能存在粪口传播。
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