Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005185.
Strain NIBR12 (=KACC 22094=HAMBI 3739), a novel Gram-stain-negative, obligate aerobic, non-spore-forming, non-motile and coccobacillus-shaped bacterium, was isolated from a cyanobacterial sample culture ( NIBRCYC000000452). The newly identified bacterial strain grew optimally in modified Reasoner's 2A medium under the following conditions: 0 % (w/v) NaCl, pH 7.5 and 35 °C. Phylogenetic analysis using the 16S rRNA gene sequence confirmed that strain NIBR12 belongs to the genus , with its closest neighbours being SHET (98.8%), RB-3 (97.7%), " R-30 (95.7 %) and H-8 (95.0 %). Genomic comparison of strain NIBR12 with type species in the genus was conducted using digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity analyses, resulting in values of ≤53.7, ≤93.7 and ≤96.1 %, respectively. The genomic DNA G+C content of strain NIBR12 was 70.9 mol%. The major fatty acids of strain NIBR12 were summed feature 8 (C 7 and/or C 6) and summed feature 3 (C 6/C 7). Q-9 was its major respiratory quinone. Moreover, the major polar lipids of strain NIBR12 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Based on our chemotaxonomic, genotypic and phenotype analyses, strain NIBR12 is identified as represeting a novel species of the genus , for which the name sp. nov. is proposed.
菌株 NIBR12（=KACC 22094=HAMBI 3739），是一种新型革兰氏阴性、需氧、非孢子形成、非运动、球形短杆菌，从蓝藻样品培养物（NIBRCYC000000452）中分离得到。新鉴定的细菌菌株在改良的 Reasoner 2A 培养基中最佳生长，条件如下：0%（w/v）NaCl、pH 7.5 和 35°C。基于 16S rRNA 基因序列的系统发育分析证实，菌株 NIBR12 属于 属，与最接近的近缘种为 SHET（98.8%）、RB-3（97.7%）、“R-30”（95.7%）和 H-8（95.0%）。通过数字 DNA-DNA 杂交、平均核苷酸同一性和平均氨基酸同一性分析，与属内模式种进行了菌株 NIBR12 的基因组比较，结果分别为≤53.7、≤93.7 和≤96.1%。菌株 NIBR12 的基因组 DNA G+C 含量为 70.9mol%。菌株 NIBR12 的主要脂肪酸为总和特征 8（C7 和/或 C6）和总和特征 3（C6/C7）。Q-9 是其主要呼吸醌。此外，菌株 NIBR12 的主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。基于我们的化学分类、基因型和表型分析，菌株 NIBR12 代表了属的一个新型种，建议将其命名为 sp. nov.。
