Sminia T, Wilders M M, Janse E M, Hoefsmit E C
Immunobiology. 1983 Mar;164(2):136-43. doi: 10.1016/S0171-2985(83)80005-9.
The aim of the present study was to correlate in vitro and in situ observations on nonlymphoid cells in Peyer's patches (PP) of the rat. By carrying out enzyme cytochemical reactions (acid phosphatase, APh, and non-specific esterase, NSE) and immunocytochemistry (Ia antigen staining) on cell suspensions and cryostat sections of PP, two classes of nonlymphoid cells could be distinguished. These were (1) strongly APh- and NSE-positive cells without or with a slight Ia membrane staining and (2) strongly Ia-positive cells with a weak APh and NSE activity. The first cell class comprised the classical macrophages which, except for tingible body macrophages, were glass-adherent. The second cell class was non-adherent and comprised dendritic (interdigitating) cells. The role of this Ia-positive non-adherent cell population was discussed, and a hypothesis was presented on the relation between mononuclear blood cells, veiled cells, and interdigitating cells in PP.
本研究的目的是将大鼠派伊尔结（PP）中非淋巴细胞的体外和原位观察结果进行关联。通过对PP的细胞悬液和低温切片进行酶细胞化学反应（酸性磷酸酶，APh，和非特异性酯酶，NSE）以及免疫细胞化学（Ia抗原染色），可区分出两类非淋巴细胞。它们分别是：（1）APh和NSE呈强阳性的细胞，无Ia膜染色或仅有轻微Ia膜染色；（2）Ia呈强阳性的细胞，APh和NSE活性较弱。第一类细胞包括经典巨噬细胞，除了含铁血黄素巨噬细胞外，这些巨噬细胞可黏附于玻片。第二类细胞不黏附，包括树突状（交错突）细胞。讨论了这种Ia阳性非黏附细胞群体的作用，并提出了关于PP中单核血细胞、面纱细胞和交错突细胞之间关系的假说。
