Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Korea.
Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.
Int J Mol Sci. 2022 Feb 17;23(4):2221. doi: 10.3390/ijms23042221.
C2 domain-containing proteins (C2DPs) have been identified in different genomes that contain single or multiple C2 domains in their C- or N-terminal. It possesses higher functional activity in the transmembrane regions. The identification of C2 domains were reported in a previous study, such as multiple C2 domains and transmembrane-region proteins (MCTPs) and N-terminal-TM-C2 domain proteins (NTMC2s) of rice, , and cotton, whereas the gene family in rice has not been comprehensively studied, and the role of the gene in rice in response to abiotic stress is not yet fully understood. In this study, we identified 82 C2DPs in the rice genome and divided them into seven groups through phylogenetic analysis. The synteny analysis revealed that duplication events were either exhibited within the genome of rice or between the genomes of rice and other species. Through the analysis of -acting elements in promoters, expression profiles, and qRT-PCR results, the functions of were found to be widely distributed in diverse tissues and were extensively involved in phytohormones-related and abiotic stresses response in rice. The prediction of the microRNA (miRNA) targets of revealed the possibility of regulation by consistent miRNAs. Notably, as a co-tandem duplication exhibited similar expression variations and involved the coincident miRNA-regulation pathway. Moreover, the results of the genotypic variation and haplotype analysis revealed that , , and were associated with cold stress responses. These findings provided comprehensive insights for characterizations of in rice as well as for their roles for abiotic stress.
C2 结构域蛋白（C2DPs）在不同的基因组中被发现，它们在 C 端或 N 端含有单个或多个 C2 结构域。它在跨膜区域具有更高的功能活性。C2 结构域的鉴定在以前的研究中已经有报道，如水稻中的多个 C2 结构域和跨膜区域蛋白（MCTPs）和 N 端-TM-C2 结构域蛋白（NTMC2s），而水稻中的 基因家族尚未得到全面研究，该基因在水稻应对非生物胁迫中的作用尚不完全清楚。在本研究中，我们在水稻基因组中鉴定了 82 个 C2DPs，并通过系统发育分析将它们分为七个组。共线性分析表明，在水稻基因组内或在水稻和其他物种的基因组之间都发生了复制事件。通过对启动子中的 - 顺式作用元件、表达谱和 qRT-PCR 结果的分析，发现 的功能广泛分布在不同的组织中，并广泛参与了水稻中与植物激素相关的非生物胁迫反应。对 的 microRNA（miRNA）靶标的预测表明了一致的 miRNA 调节的可能性。值得注意的是，作为一个共串联重复，表现出相似的表达变化，并涉及一致的 miRNA 调控途径。此外，基因型变异和单倍型分析的结果表明， 、 和 与冷胁迫反应有关。这些发现为水稻中 的特征以及它们在非生物胁迫中的作用提供了全面的见解。
