Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Trends Biochem Sci. 2021 Nov;46(11):918-930. doi: 10.1016/j.tibs.2021.05.010. Epub 2021 Jul 8.
Single-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and transient binding of fluorescently labeled DNA probes. In its early stages the implementation of DNA-PAINT was burdened by low-throughput, excessive acquisition time, and difficult integration with live-cell imaging. However, recent advances are addressing these challenges and expanding the range of applications of DNA-PAINT. We review the current state of the art of DNA-PAINT in light of these advances and contemplate what further developments remain indispensable to realize live-cell imaging.
单分子定位显微镜（SMLM）是一种强大的工具，可用于以前所未有的分辨率检查生物系统，使越来越小的结构的研究成为可能。在这些发展的最前沿是基于 DNA 的点积累用于纳米级形貌的成像（DNA-PAINT），它利用荧光标记的 DNA 探针的随机和瞬时结合。在早期，DNA-PAINT 的实施受到低通量、过长的采集时间和与活细胞成像难以集成的困扰。然而，最近的进展正在解决这些挑战，并扩大 DNA-PAINT 的应用范围。我们根据这些进展回顾了 DNA-PAINT 的最新技术，并思考了实现活细胞成像还需要哪些进一步的发展。
