Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
Department of Nephrology, First Hospital of Jilin University, Changchun, China.
Front Immunol. 2021 Jun 23;12:698494. doi: 10.3389/fimmu.2021.698494. eCollection 2021.
induced alternative activated macrophages (M2), leading to protect against Crohn's disease, known as Th1 -related inflammation, which enhances oxidative stress in the host. However, the relationship of oxidative stress and -mediated immune response is still unknown. In our study, we showed that nuclear factor erythroid 2-related factor-2 (Nrf2), a key transcription factor in antioxidant, participated in M2 polarization induced by muscle larval excretory/secretory (ES) products . ES -treated M2 were injected intravenously after TNBS challenge and we demonstrated that ES-M could alleviate the severity of the colitis in mice. Adoptive transfer of ES -treated M2 decreased the level of IFN-γ and increased the levels of IL-4 and IL-10 . However, the capacity of ES -treated Nrf2 KO macrophages to treat colitis was dramatically impaired. ES -treated Nrf2 KO macrophages was insufficient to result in the elevated levels of IL-4 and IL-10. These findings indicate that Nrf2 was required for M2 polarization induced by ES to alleviate colitis in mice.
诱导替代激活的巨噬细胞（M2），从而预防克罗恩病，称为 Th1 相关炎症，这会增强宿主的氧化应激。然而，氧化应激与 - 介导的免疫反应之间的关系尚不清楚。在我们的研究中，我们表明核因子红细胞 2 相关因子 2（Nrf2），一种抗氧化的关键转录因子，参与了 - 肌肉幼虫排泄/分泌（ES）产物诱导的 M2 极化。ES 处理后的 M2 在 TNBS 挑战后静脉注射，我们证明 ES-M 可以减轻小鼠结肠炎的严重程度。ES 处理的 M2 的过继转移降低了 IFN-γ 的水平，增加了 IL-4 和 IL-10 的水平。然而，ES 处理的 Nrf2 KO 巨噬细胞治疗结肠炎的能力显著受损。ES 处理的 Nrf2 KO 巨噬细胞不足以导致 IL-4 和 IL-10 的水平升高。这些发现表明，Nrf2 是 ES 诱导的 M2 极化减轻小鼠结肠炎所必需的。
