近紫外辐射与过氧化氢协同杀灭大肠杆菌：RecA可修复损伤与RecA不可修复损伤之间的区别
Synergistic killing of Escherichia coli by near-UV radiation and hydrogen peroxide: distinction between recA-repairable and recA-nonrepairable damage.
作者信息
Hartman P S, Eisenstark A
出版信息
J Bacteriol. 1978 Feb;133(2):769-74. doi: 10.1128/jb.133.2.769-774.1978.
Abstract
Wild-type cells and six DNA repair-deficient mutants (lexA, recA, recB, recA, recB, polA1, and uvrA) of Escherichia coli K-12 were treated with near-ultraviolet radiation plus hydrogen peroxide (H2O2). At low H2O2 concentrations (6 X 10(-6) to 6 X 10(-4) M), synergistic killing occurred in all strains except those containing a mutation in recA. This RecA-repairable damage was absent from stationary-phase cells but increased in logarithmic cells as a function of growth rate. At higher H2O2 concentrations (above 6 X 10(-4) M) plus near-ultraviolet radiation, all strains, including those with a mutation in recA, were synergistically killed; thus, at high H2O2 concentrations, the damage was not RecA repairable.
摘要
用近紫外辐射加过氧化氢（H₂O₂）处理大肠杆菌K - 12的野生型细胞和六个DNA修复缺陷突变体（lexA、recA、recB、recA、recB、polA1和uvrA）。在低H₂O₂浓度（6×10⁻⁶至6×10⁻⁴M）下，除含有recA突变的菌株外，所有菌株均出现协同杀伤作用。这种可被RecA修复的损伤在稳定期细胞中不存在，但在对数期细胞中随生长速率增加。在较高H₂O₂浓度（高于6×10⁻⁴M）加近紫外辐射时，所有菌株，包括那些含有recA突变的菌株，均被协同杀伤；因此，在高H₂O₂浓度下，损伤不能被RecA修复。
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本文引用的文献
1
Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B".
Proc Natl Acad Sci U S A. 1946 May;32(5):120-8. doi: 10.1073/pnas.32.5.120.
2
Catalase, hydrogen peroxide, and ionizing radiation.
Radiat Res. 1963;Suppl 3:110-29.
3
Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
Genetics. 1966 Jun;53(6):1119-36. doi: 10.1093/genetics/53.6.1119.
4
Some observations on the lethal effects of near-ultraviolet light on Escherichia coli, compared with the lethal effects of far-ultraviolet light.
Photochem Photobiol. 1970 Jul;12(1):1-8. doi: 10.1111/j.1751-1097.1970.tb06031.x.
5
Isolation of an E. coli strain with a mutation affecting DNA polymerase.
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
6
The beginning of a genetic analysis of recombination proficiency.
J Cell Physiol. 1967 Oct;70(2):Suppl:165-80. doi: 10.1002/jcp.1040700412.
7
Survival of bacteria. Harmful effects of light, with some comparisons with other adverse physical agents.
Annu Rev Microbiol. 1967;21:143-56. doi: 10.1146/annurev.mi.21.100167.001043.
8
An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants.
Proc Natl Acad Sci U S A. 1974 May;71(5):1838-42. doi: 10.1073/pnas.71.5.1838.
9
Reduced dimer excision in bacteria following near ultraviolet (365 nm) radiation.
Mutat Res. 1973 Sep;19(3):361-4. doi: 10.1016/0027-5107(73)90238-8.
10
The synergistic action of ultraviolet and x radiation on mutants of Escherichia coli K-12.
Photochem Photobiol. 1973 Jul;18(1):1-8. doi: 10.1111/j.1751-1097.1973.tb06385.x.
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