使用API 20E系统鉴定肠杆菌科细菌。
Identification of Enterobacteriaceae by the API 20E system.
作者信息
Holmes B, Willcox W R, Lapage S P
出版信息
J Clin Pathol. 1978 Jan;31(1):22-30. doi: 10.1136/jcp.31.1.22.
Abstract
Since the introduction of the API 20E kit a number of identification schemes have been developed by the manufacturer for use with the kit. We evaluated the success of these various schemes in identifying 206 strains belonging to 34 taxa of the family Enterobacteriaceae. Many of the strains were atypical and only 94% could be identified by our own system of 50 conventional tests and a computer program. The most advanced identification scheme so far developed for the API 20E kit (the Analytical Profile Index and complementary Computer Service) allowed 88% of the 206 strains to be correctly identified, although 2% were incorrectly identified. The tests in the API 20E kit and 52 conventional tests were separately evaluated for their ability to discriminate between the 34 taxa considered in this study. Our results suggest that replacing some of the tests in the present API 20E kit might further improve its diagnostic performance.
摘要
自从API 20E试剂盒推出以来，制造商已经开发了许多与该试剂盒配套使用的鉴定方案。我们评估了这些不同方案在鉴定属于肠杆菌科34个分类单元的206株菌株方面的成功率。许多菌株是非典型的，通过我们自己的50项传统测试和一个计算机程序，只有94%的菌株能够被鉴定出来。迄今为止为API 20E试剂盒开发的最先进的鉴定方案（分析谱指数和配套计算机服务）能够正确鉴定206株菌株中的88%，尽管有2%被错误鉴定。分别评估了API 20E试剂盒中的测试和52项传统测试区分本研究中所考虑的34个分类单元的能力。我们的结果表明，替换当前API 20E试剂盒中的一些测试可能会进一步提高其诊断性能。
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