Tian Fenghua, Li Changtian, Li Yu
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China.
Front Microbiol. 2021 Jul 1;12:652324. doi: 10.3389/fmicb.2021.652324. eCollection 2021.
Yuanmo [ (Y.C. Dai, Niemelä & G.F. Qin) T. Saito, Tonouchi & T. Harada] is an important edible and medicinal mushroom endemic to Northeastern China. Here we report the sequencing and assembly of the genome using single-molecule real-time sequencing technology. The whole genome was approximately 35.65 Mb, with a G + C content of 48.31%. Genome assembly generated 41 contigs with an N50 length of 1,772,559 bp. The genome comprised 9,364 annotated protein-coding genes, many of which encoded enzymes involved in the modification, biosynthesis, and degradation of glycoconjugates and carbohydrates or enzymes predicted to be involved in the biosynthesis of secondary metabolites such as terpene, type I polyketide, siderophore, and fatty acids, which are responsible for the pharmacodynamic activities of . We also identified genes encoding 1,3-β-glucan synthase and endo-1,3(4)-β-glucanase, which are involved in polysaccharide and uridine diphosphate glucose biosynthesis. Phylogenetic and comparative analyses of Basidiomycota fungi based on a single-copy orthologous protein indicated that the genus is an independent group that evolved from the Pleurotaceae family. The annotated whole-genome sequence of can serve as a reference for investigations of bioactive compounds with medicinal value and the development and commercial production of superior varieties.
元蘑[（戴玉成、涅梅拉和秦桂芳）齐藤敏郎、户野内和原田彻]是中国东北地区特有的一种重要食药用真菌。在此，我们报道了使用单分子实时测序技术对其基因组进行测序和组装的情况。其全基因组约为35.65 Mb，G + C含量为48.31%。基因组组装产生了41个重叠群，N50长度为1,772,559 bp。该基因组包含9364个注释的蛋白质编码基因，其中许多基因编码参与糖缀合物和碳水化合物修饰、生物合成及降解的酶，或预测参与萜类、I型聚酮化合物、铁载体和脂肪酸等次生代谢物生物合成的酶，这些次生代谢物负责元蘑的药效活性。我们还鉴定出了编码1,3-β-葡聚糖合酶和内切-1,3(4)-β-葡聚糖酶的基因，它们参与多糖和尿苷二磷酸葡萄糖的生物合成。基于单拷贝直系同源蛋白对担子菌纲真菌进行的系统发育和比较分析表明，元蘑属是一个从侧耳科进化而来的独立类群。元蘑的全基因组序列注释可为具有药用价值的生物活性化合物的研究以及优良元蘑品种的开发和商业化生产提供参考。
