Yuan Xiaoxiao, Peng Keqin, Li Changtian, Zhao Zhibo, Zeng Xiangyu, Tian Fenghua, Li Yu
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
J Fungi (Basel). 2021 Aug 28;7(9):707. doi: 10.3390/jof7090707.
"Hongtuozhusun" () is an important edible and medicinal mushroom endemic to Southwest China. However, yellow rot disease is a severe disease of . that occurs extensively in Guizhou Province. It has caused major economic losses and hinders the development of the . industry. In this study, 28 microorganism strains were isolated from diseased fruiting bodies of at various stages, two of which were confirmed to be pathogenic based on Koch's postulates. These two strains are introduced herein as sp. nov. based on morphological, physiological, and molecular analysis. We reported a high-quality de novo sequencing and assembly of the . genome using single-molecule real-time sequencing technology. The whole genome was approximately 14.148 Mb with a G+C content of 43.55%. Genome assembly generated 8 contigs with an N50 length of 1,822,654 bp. The genome comprised 5966 annotated protein-coding genes. This is the first report of mushroom disease caused by species. We expect that the information on genome properties, particularly in pathogenicity-related genes, assist in developing effective control measures in order to prevent severe losses and make amendments in management strategies.
“红托竹荪”（）是中国西南地区特有的一种重要食药用菌。然而，黄腐病是其一种严重病害，在贵州省广泛发生。它已造成重大经济损失并阻碍了该产业的发展。在本研究中，从处于不同阶段的患病子实体中分离出28株微生物菌株，其中两株根据柯赫氏法则被确认为病原菌。基于形态学、生理学和分子分析，这两株菌株在此被命名为新种。我们报道了使用单分子实时测序技术对该基因组进行的高质量从头测序和组装。全基因组约为14.148 Mb，G+C含量为43.55%。基因组组装产生了8个重叠群，N50长度为1,822,654 bp。该基因组包含5966个注释的蛋白质编码基因。这是关于由该物种引起的蘑菇病害的首次报道。我们期望关于基因组特性的信息，特别是与致病性相关的基因，有助于制定有效的控制措施，以防止严重损失并调整管理策略。
