Lai Ningyu, Luo Yuanchan, Fei Peng, Hu Peng, Wu Hui
State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Shanghai GTL Biotech Co., Ltd., 1688 North Guoquan Road, Shanghai, 200438, China.
Synth Syst Biotechnol. 2021 Jun 29;6(3):144-152. doi: 10.1016/j.synbio.2021.06.003. eCollection 2021 Sep.
Syngas, which contains large amount of CO as well as H and CO, can be convert to acetic acid chemically or biologically. Nowadays, acetic acid become a cost-effective nonfood-based carbon source for value-added biochemical production. In this study, acetic acid and CO were used as substrates for the biosynthesis of 3-hydroxypropionic acid (3-HP) in metabolically engineered carrying heterogeneous acetyl-CoA carboxylase (Acc) from and codon-optimized malonyl-CoA reductase (MCR) from . Strategies of metabolic engineering included promoting glyoxylate shunt pathway, inhibiting fatty acid synthesis, dynamic regulating of TCA cycle, and enhancing the assimilation of acetic acid. The engineered strain LNY07(MDA) accumulated 15.8 g/L of 3-HP with the yield of 0.71 g/g in 48 h by whole-cell biocatalysis. Then, syngas-derived acetic acid was used as substrate instead of pure acetic acid. The concentration of 3-HP reached 11.2 g/L with the yield of 0.55 g/g in LNY07(MDA). The results could potentially contribute to the future development of an industrial bioprocess of 3-HP production from syngas-derived acetic acid.
合成气含有大量的一氧化碳以及氢气和一氧化碳，可以通过化学或生物方法转化为乙酸。如今，乙酸已成为一种具有成本效益的非食品基碳源，用于生产高附加值的生物化学品。在本研究中，乙酸和一氧化碳被用作代谢工程菌株中3-羟基丙酸（3-HP）生物合成的底物，该菌株携带来自[具体来源1]的异源乙酰辅酶A羧化酶（Acc）和来自[具体来源2]的密码子优化的丙二酰辅酶A还原酶（MCR）。代谢工程策略包括促进乙醛酸循环途径、抑制脂肪酸合成、动态调节三羧酸循环以及增强乙酸的同化作用。工程菌株LNY07（MDA）通过全细胞生物催化在48小时内积累了15.8 g/L的3-HP，产率为0.71 g/g。然后，将合成气衍生的乙酸用作底物，而不是纯乙酸。在LNY07（MDA）中，3-HP的浓度达到11.2 g/L，产率为0.55 g/g。这些结果可能有助于未来从合成气衍生的乙酸生产3-HP的工业生物过程的发展。
