琼脂稀释法与Sceptor厌氧菌MIC/ID微量肉汤稀释板测定厌氧菌药敏结果的比较。

Comparison of susceptibility results of anaerobic organisms determined by agar dilution method and Sceptor Anaerobe MIC/ID Micro Broth Dilution Panels.

作者信息

Hussain Z, Lannigan R, Schieven B C, Stoakes L, Groves D

机构信息

Department of Clinical Microbiology, Victoria Hospital Corporation, London, Ontario, Canada.

出版信息

Diagn Microbiol Infect Dis. 1987 Oct;8(2):95-100. doi: 10.1016/0732-8893(87)90155-6.

DOI:10.1016/0732-8893(87)90155-6

PMID:3427890

Abstract

A commercial broth microdilution system (Sceptor Anaerobe MIC/ID, BBL) for susceptibility testing of anaerobic bacteria was compared to a reference agar dilution system. Of the 172 organisms tested, only 7% failed to grow sufficiently for testing in the Sceptor system. In 1,590 antibiotic/organism combinations, 86.7% of the Sceptor results were identical or within one doubling dilution of the reference system. In 91.9% of the cases, interpretation of the results was same in both systems. Two hundred and twelve MIC values, however, differ by greater than or equal to 2 log2 dilution from the reference system. Due to this reduced correlation in the actual MIC values with the reference results, further studies are warranted before the Sceptor system can be recommended for routine use.

摘要

将一种用于厌氧菌药敏试验的商业肉汤微量稀释系统（Sceptor厌氧菌MIC/ID，BBL）与参考琼脂稀释系统进行了比较。在测试的172种微生物中，只有7%在Sceptor系统中生长不足，无法进行测试。在1590种抗生素/微生物组合中，Sceptor系统的结果有86.7%与参考系统相同或在参考系统的一个稀释倍数范围内。在91.9%的病例中，两个系统对结果的解释相同。然而，有212个MIC值与参考系统相差大于或等于2个log2稀释倍数。由于实际MIC值与参考结果之间的相关性降低，在推荐Sceptor系统用于常规使用之前，有必要进行进一步研究。