Wexler H M, Molitoris E, Murray P R, Washington J, Zabransky R J, Edelstein P H, Finegold S M
Research Service, Veterans Affairs Medical Center West Los Angeles, California, USA.
J Clin Microbiol. 1996 Jan;34(1):170-4. doi: 10.1128/jcm.34.1.170-174.1996.
A multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (SGE) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the MICs obtained by the SGE method with those obtained by the reference agar dilution method of the National Committee for Clinical Laboratory Standards. The standard deviation of the MIC obtained by the SGE method for the five participating laboratories was +/- 0.26 of a twofold dilution, whereas it was +/- 1 twofold dilution by the reference method. The interlaboratory reproducibility of the results for two control strains tested with imipenem, chloramphenicol, and metronidazole indicated that 96% of the measurements fell within +/- 1 twofold dilution of the mode. The equivalence of the SGE method with the agar dilution method was assessed with a wide variety of anaerobic organisms. The MICs by both methods were within 1 doubling dilution in 93% of the measurements (n = 1,074). Discrepancies generally occurred with those organism-drug combinations that resulted in tailing endpoints (Fusobacterium nucleatum, 86% agreement) or in cases of light growth (Peptostreptococcus spp., 86% agreement).
开展了一项多实验室协作研究，以评估螺旋梯度终点（SGE）法在测定厌氧菌抗菌药物敏感性方面的实用性，并评估通过SGE法获得的最低抑菌浓度（MIC）与通过美国国家临床实验室标准委员会的参考琼脂稀释法获得的MIC的等效性。五个参与实验室通过SGE法获得的MIC的标准差为两倍稀释度的±0.26，而参考方法为±1两倍稀释度。用亚胺培南、氯霉素和甲硝唑检测的两种对照菌株结果的实验室间再现性表明，96%的测量值落在众数的±1两倍稀释度范围内。使用多种厌氧生物评估了SGE法与琼脂稀释法的等效性。两种方法的MIC在93%的测量值（n = 1074）中在1倍稀释度范围内。差异通常发生在导致拖尾终点的微生物-药物组合（具核梭杆菌，一致性为86%）或生长较弱的情况下（消化链球菌属，一致性为86%）。
