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没食子诃子和百里香水醇提物对正常和氧化应激条件下公羊鲜精的影响。

Effects of hydroethanolic extracts of Terminalia chebula and Thymbra spicata on ram fresh semen under normal and oxidative stress conditions.

机构信息

Basic Sciences and Pathobiology Department, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.

出版信息

Vet Med Sci. 2021 Sep;7(5):1778-1785. doi: 10.1002/vms3.580. Epub 2021 Jul 20.

DOI:10.1002/vms3.580
PMID:34288575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8464289/
Abstract

The objective of this study was to evaluate protective effects of hydroethanolic extracts of Terminalia chebula and Thymbra spicata on viability, lipid peroxidation (LPO) and DNA integrity of ram fresh semen under normal and oxidative stress (OS) conditions. Antioxidant activities of different concentrations of Terminalia chebula and Thymbra spicata extracts were evaluated with DPPH assay. Semen samples were taken from three fertile adult rams. After diluting semen with Tris-base extender, different concentrations of Terminalia chebula and Thymbra spicata (30, 300, and 3000 μg/ml) extracts were used under normal and induced OS conditions. The group not receiving any supplements was considered as control group. A total of 50 μM hydrogen peroxide was used to induce OS. MTT solution was added to each of treatment groups which were kept in an incubator at 37°C for 2 h. After incubation, readings were obtained by ELISA reader. DNA integrity and LPO were determined with acridine orange (AO) staining and malondialdehyde (MDA) assay. Higher concentrations of Terminalia chebula and Thymbra spicata extracts preserved viability and DNA integrity while reducing MDA concentrations compared to other treatment groups. Also, under induced OS, higher concentrations of both extracts reduced detrimental effects of H O . In conclusion, it seems that addition of Terminalia chebula and Thymbra spicata extracts can reduce induced OS in spermatozoa.

摘要

本研究旨在评估诃子和百里香的水醇提取物对正常和氧化应激（OS）条件下公羊鲜精活力、脂质过氧化（LPO）和 DNA 完整性的保护作用。采用 DPPH 法评估不同浓度诃子和百里香提取物的抗氧化活性。从 3 只成年、健康公羊采集精液样品。用 Tris 基础稀释液稀释精液后，在正常和诱导 OS 条件下，使用不同浓度的诃子和百里香（30、300 和 3000μg/ml）提取物。不接受任何补充剂的组被认为是对照组。用 50μM 过氧化氢诱导 OS。将 MTT 溶液添加到每个处理组中，在 37°C 的孵育箱中孵育 2 小时。孵育后，通过 ELISA 读取器获得读数。通过吖啶橙（AO）染色和丙二醛（MDA）测定法测定 DNA 完整性和 LPO。与其他处理组相比，较高浓度的诃子和百里香提取物可保存活力和 DNA 完整性，同时降低 MDA 浓度。此外，在诱导 OS 下，两种提取物的较高浓度均可减少 H O 对精子的有害影响。总之，添加诃子和百里香提取物可以减少精子中的诱导 OS。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/866ab68150f9/VMS3-7-1778-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/04911d0668ea/VMS3-7-1778-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/8087c89336d4/VMS3-7-1778-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/7a164166d4b8/VMS3-7-1778-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/fbd8fb04627d/VMS3-7-1778-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/866ab68150f9/VMS3-7-1778-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/04911d0668ea/VMS3-7-1778-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/8087c89336d4/VMS3-7-1778-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/7a164166d4b8/VMS3-7-1778-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/fbd8fb04627d/VMS3-7-1778-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/867b/8464289/866ab68150f9/VMS3-7-1778-g006.jpg

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