Suresh Kumar Devadharshini, Alex Arockia, Neha Brahma, R Kalpana, Vimal Sugumar
Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, IND.
Department of Bioengineering, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, IND.
Cureus. 2024 Jul 5;16(7):e63930. doi: 10.7759/cureus.63930. eCollection 2024 Jul.
Aim This study aims to investigate the antibacterial, antifungal, and phytochemical properties of methanolic tuber extracts from . Additionally, the study seeks to assess the in vitro anticancer effects of these extracts on an oral cancer cell line, as well as their antioxidant and anti-inflammatory activities. Materials and methods The research involves examining the antibacterial and antifungal properties of methanolic tuber extracts from . The phytochemical composition will be analyzed using standard techniques. The in vitro anticancer effects will be tested on an oral cancer cell line, while antioxidant and anti-inflammatory activities will be evaluated through appropriate assays. Results The study demonstrated that methanolic tuber extracts exhibit cytotoxic effects on the oral cancer cell line (KB-1), reducing cell viability as evidenced by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A concentration of 30 µg/mL induced notable morphological changes observed under an inverted fluorescence microscope. Antioxidant assays showed a maximum absorption of 85.3% with 50 µL of the extract, while anti-inflammatory tests revealed a 76.0% absorption. Antimicrobial activity, assessed via agar-well diffusion, indicated significant antibacterial effects, especially against and at higher concentrations. The findings suggest promising therapeutic potential for extracts. Conclusion tuber extracts may treat diseases caused by studied organisms. The study suggests that methanolic extracts from tubers have potential commercial value due to their anti-inflammatory, antioxidant, and cytotoxic properties. The extracts induced apoptosis in an oral cancer cell line at 30 µg/mL after 24 hours. Further research is needed to understand the active components and underlying molecular mechanisms.
目的 本研究旨在调查[植物名称]块茎甲醇提取物的抗菌、抗真菌和植物化学特性。此外，该研究还试图评估这些提取物对口腔癌细胞系的体外抗癌作用，以及它们的抗氧化和抗炎活性。材料与方法 该研究包括检测[植物名称]块茎甲醇提取物的抗菌和抗真菌特性。将使用标准技术分析植物化学成分。将在口腔癌细胞系上测试体外抗癌作用，同时通过适当的测定评估抗氧化和抗炎活性。结果 研究表明，[植物名称]块茎甲醇提取物对口腔癌细胞系（KB-1）表现出细胞毒性作用，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法证明细胞活力降低。30μg/mL的浓度在倒置荧光显微镜下观察到明显的形态变化。抗氧化测定显示，50μL提取物的最大吸光度为85.3%，而抗炎测试显示吸光度为76.0%。通过琼脂孔扩散评估的抗菌活性表明有显著的抗菌作用，尤其是在较高浓度下对[细菌名称1]和[细菌名称2]。这些发现表明[植物名称]提取物具有潜在的治疗潜力。结论 [植物名称]块茎提取物可能治疗由所研究生物体引起的疾病。该研究表明，[植物名称]块茎的甲醇提取物因其抗炎、抗氧化和细胞毒性特性而具有潜在的商业价值。24小时后，30μg/mL的提取物在口腔癌细胞系中诱导细胞凋亡。需要进一步研究以了解活性成分和潜在的分子机制。
