Yuan Shuai, Li Qingshu, Chen Kaiwen, Mu Zhixiang, Chen Tao, Wang Huanan, Ji Ping
Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, 401147, P. R. China.
Department of Pathology, Chongqing Medical University, Chongqing, 401147, P. R. China.
J Biol Eng. 2021 Jul 21;15(1):19. doi: 10.1186/s13036-021-00271-8.
Ridge preservation is universally acknowledged as the conventional method for the post-extraction healing yet there are no standard materials for the ideal healing outcome. Herein, a composite gel comprising gelatin nanoparticles (GNPs) and injectable platelet-rich-fibrin (i-PRF) as the potential candidate for extracted socket healing is introduced. The combination of GNPs and i-PRF not only possesses favorable mechanical properties to withstand external force but also accelerate the blood clotting time significantly. In addition, six beagle dogs were adopted to assess the angiogenic and osteogenic capacity of GNPs+i-PRF gel in vivo. The GNPs+i-PRF gel significantly produced the most blood vessels area, woven bone and low osteoclast activity in extracted sockets at 2 weeks postoperation and remarkably generated corticalization on the alveolar ridge crest at 8 weeks postoperation according to histological results. Therefore, GNPs+i-PRF gel can be recommended as the candidate grafting material regarding ridge preservation for its cost effectiveness, excellent biocompatibility, facilitation of blood clotting and favorable capacity of promoting angiogenesis and osteogenesis.
牙槽嵴保存被公认为拔牙后愈合的传统方法，但目前尚无用于实现理想愈合效果的标准材料。在此，介绍一种由明胶纳米颗粒（GNPs）和可注射富血小板纤维蛋白（i-PRF）组成的复合凝胶，作为拔牙窝愈合的潜在候选材料。GNPs和i-PRF的组合不仅具有良好的机械性能以承受外力，还能显著加快凝血时间。此外，采用6只比格犬评估GNPs+i-PRF凝胶在体内的血管生成和成骨能力。组织学结果显示，GNPs+i-PRF凝胶在术后2周时在拔牙窝内显著产生了最大的血管面积、编织骨且破骨细胞活性较低，在术后8周时在牙槽嵴顶显著形成了皮质化。因此，GNPs+i-PRF凝胶因其成本效益、优异的生物相容性、促进凝血以及促进血管生成和成骨的良好能力，可被推荐作为牙槽嵴保存的候选移植材料。
