Yi Ke, Li Qing, Lian Xiaodong, Wang Yapei, Tang Zhihui
Second Clinical Division, Peking University School and Hospital of Stomatology, Beijing 100101, China.
National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China.
Regen Biomater. 2022 Apr 13;9:rbac021. doi: 10.1093/rb/rbac021. eCollection 2022.
Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials. Although the injectable platelet-rich fibrin (i-PRF) is a safe, autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue, its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors. We herein sought to develop a bioactive bioink composed of i-PRF, alginate and gelatin capable of promoting the regeneration of the oral soft tissue. This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs. Importantly, we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF. The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts. When deployed in nude mice, this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity. Together, these data thus confirm the successful production of an i-PRF-containing bioink, which is suitable for the individualized promotion of the regeneration of oral soft tissue.
由于现有治疗材料的疗效有限，口腔软组织缺损仍然难以治疗。尽管可注射富血小板纤维蛋白（i-PRF）是一种安全的、富含高水平生长因子的自体来源材料，常用于促进口腔软组织的再生，但其效果受到术中塑形困难以及生长因子爆发式释放的限制。我们在此试图开发一种由i-PRF、藻酸盐和明胶组成的生物活性生物墨水，能够促进口腔软组织的再生。这种生物墨水成功应用于3D生物打印，并展现出根据个体患者需求进行塑形的能力。重要的是，与i-PRF相比，我们还能够显著延长多种生长因子的释放时间。打印的牙龈成纤维细胞增殖和活力增强进一步证实了生长因子的生物利用度。当植入裸鼠体内时，这种生物墨水进一步被证实具有生物相容性，并能促进血管生成活性增强。总之，这些数据证实了含i-PRF生物墨水的成功制备，其适用于个性化促进口腔软组织再生。
