Yabe Shuhei, Zheng Yu, Wang Chiung-Mei, Sakai Yasuteru, Abe Keietsu, Yokota Akira, Donadio Stefano, Cavaletti Linda, Monciardini Paolo
Department of Microbial Resources, Graduate School of Agricultural Sciences, Tohoku University, Sendai, Miyagi 980-0845, Japan.
Hazaka Plant Research Center, Kennan Eisei Kogyo Co. Ltd., Miyagi 989-1311, Japan.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004883.
The aerobic, Gram-positive, mesophilic strains, Uno17, SOSP1-1, 1-9, 1-30 and 150040, formed mycelia of irregularly branched filaments, produced spores or sporangia, and numerous secondary metabolite biosynthetic gene clusters. The five strains grew at 15-40 °C (optimally at 30 °C) and pH 4.0-8.0 (optimally at pH 6.0-7.0), and had 7.21-12.67 Mb genomes with 49.7-53.7 mol% G+C content. They shared MK9(H) as the major menaquinone and C-2OH and iso-C as the major cellular fatty acids. Phylogenetic and phylogenomic analyses showed that Uno17 and SOSP1-9 were most closely related to members of the genus , with 94.43-96.21 % 16S rRNA gene similarities and 72.16-81.56% genomic average nucleotide identity. The strain most closely related to SOSP1-1 and SOSP1-30 was SOSP1-21, with 91.33 and 98.84 % 16S rRNA similarities, and 75.13 and 92.35% average nucleotide identities, respectively. Strain 150040 formed a distinct clade within the order , showing <90.47 % 16S rRNA gene similarity to known species in this order. Based on these results, we propose: strain 150040 as gen. nov., sp. nov. (type strain 150 040=CGMCC 1.17052=BCRC 81202) within the family fam. nov. in the order ; strain SOSP1-1 as gen. nov., sp. nov. (type strain SOSP1-1=CGMCC 1.17205=BCRC 81203) and strain SOSP1-30 as sp. nov. (type strain SOSP1-30=CGMCC 1.17733=BCRC 81205) within the family ; strain Uno17 as sp. nov. (type strain Uno17=NBRC 113155=BCRC 81116); and strain SOSP1-9 as sp. nov. (type strain SOSP1-9=CGMCC 1.17206=BCRC 81204) within the family .
需氧、革兰氏阳性、嗜温菌株Uno17、SOSP1-1、1-9、1-30和150040形成不规则分支丝状的菌丝体，产生孢子或孢子囊以及众多次生代谢物生物合成基因簇。这五株菌株在15-40 °C（最适温度为30 °C）和pH 4.0-8.0（最适pH为6.0-7.0）条件下生长，基因组大小为7.21-12.67 Mb，G+C含量为49.7-53.7 mol%。它们以MK9(H)作为主要甲基萘醌，以C-2OH和异-C作为主要细胞脂肪酸。系统发育和系统基因组分析表明，Uno17和SOSP1-9与 属的成员关系最为密切，16S rRNA基因相似度为94.43-96.21 %，基因组平均核苷酸同一性为72.16-81.56%。与SOSP1-1和SOSP1-30关系最密切的菌株是SOSP1-21，16S rRNA相似度分别为91.33%和98.84 %，平均核苷酸同一性分别为75.13%和92.35%。菌株150040在 目内形成一个独特的分支，与该目已知物种的16S rRNA基因相似度<90.47 %。基于这些结果，我们提议：将菌株150040定为 科 目 中的新属、新种（模式菌株150 040=CGMCC 1.17052=BCRC 81202）；将菌株SOSP1-1定为 科中的新属、新种（模式菌株SOSP1-1=CGMCC 1.17205=BCRC 81203），将菌株SOSP1-30定为 科中的新种（模式菌株SOSP1-30=CGMCC 1.17733=BCRC 81205）；将菌株Uno17定为 科中的新种（模式菌株Uno17=NBRC 113155=BCRC 81116）；将菌株SOSP1-9定为 科中的新种（模式菌株SOSP1-9=CGMCC 1.17206=BCRC 81204）。
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