Yabe Shuhei, Sakai Yasuteru, Abe Keietsu, Yokota Akira, Také Akira, Matsumoto Atsuko, Sugiharto Arwan, Susilowati Dwiningsih, Hamada Moriyuki, Nara Kazuhide, Made Sudiana I, Otsuka Shigeto
Graduate School of Agricultural Sciences, Tohoku University, 468-1, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-0845, Japan.
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd., 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2615-2621. doi: 10.1099/ijsem.0.001985. Epub 2017 Jul 31.
A mesophilic, Gram-stain-positive, spore-forming bacterium that formed branched mycelia was isolated from paddy soil in Gunung Salak (Mount Salak), West Java, Indonesia. This strain, designated S-27T, grew at temperatures between 20 and 37 °C; the optimum growth temperature was 25 to 30 °C, and no growth was observed at 15 or 45 °C. The pH range for growth was pH 3.5 to 8.6; the optimum pH was 6.0, and no growth was observed at pH 3.0 or 9.2. Strain S-27T was able to hydrolyse polysaccharides such as starch, cellulose and xylan. The G+C content of the DNA of strain S-27T was 55.7 mol%. The major fatty acids were iso-C17 : 0 and C16 : 1 2-OH, and the major menaquinone was MK-9 (H2). The cell wall of strain S-27T contained d-glutamic acid, glycine, l-alanine, d-alanine, l-ornithine and β-alanine in a molar ratio of 1.0 : 1.6 : 1.4 : 0.6 : 0.9 : 1.1. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol and two glycolipids. The major cell-wall sugar was arabinose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S-27T belongs to the order Ktedonobacterales and is most closely related to Ktedonobacter racemifer SOSP1-21T (89.6 % sequence identity). On the basis of its chemotaxonomic and phenotypic features and phylogenetic position, we concluded that strain S-27T represents a novel genus and species, for which we propose the name Dictyobacter aurantiacus gen. nov., sp. nov. The type strain of Dictyobacter aurantiacus is strain S-27T (=NBRC 109595T=InaCC B312T). Emendation of the description of the genus Thermosporothrix is also provided.
从印度尼西亚西爪哇省萨拉克山（Gunung Salak）的稻田土壤中分离出一株嗜温、革兰氏染色阳性、形成芽孢且产生分支菌丝体的细菌。该菌株命名为S-27T，在20至37 °C的温度范围内生长；最适生长温度为25至30 °C，在15或45 °C下未观察到生长。生长的pH范围为pH 3.5至8.6；最适pH为6.0，在pH 3.0或9.2下未观察到生长。菌株S-27T能够水解淀粉、纤维素和木聚糖等多糖。菌株S-27T的DNA的G+C含量为55.7 mol%。主要脂肪酸为异-C17 : 0和C16 : 1 2-OH，主要甲基萘醌为MK-9（H2）。菌株S-27T的细胞壁含有摩尔比为1.0 : 1.6 : 1.4 : 0.6 : 0.9 : 1.1的d-谷氨酸、甘氨酸、l-丙氨酸、d-丙氨酸、l-鸟氨酸和β-丙氨酸。极性脂质由磷脂酰甘油、磷脂酰肌醇和两种糖脂组成。主要细胞壁糖为阿拉伯糖。基于16S rRNA基因序列的详细系统发育分析表明，菌株S-27T属于Ktedonobacterales目，与消旋Ktedonobacter racemifer SOSP1-21T关系最为密切（序列同一性为89.6 %）。基于其化学分类和表型特征以及系统发育位置，我们得出结论，菌株S-27T代表一个新的属和种，我们将其命名为橙色盘杆菌（Dictyobacter aurantiacus），属名新属，种名新种。橙色盘杆菌的模式菌株是菌株S-27T（=NBRC 109595T=InaCC B312T）。还提供了对嗜热孢丝菌属描述的修订。
