Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA.
Department of Food Science, University of Tennessee, Knoxville, TN 37996, USA.
Int J Mol Sci. 2021 Jul 9;22(14):7382. doi: 10.3390/ijms22147382.
Culinary sage ( L.) is a common spice plant in the mint family (Lamiaceae) well known for its distinctive culinary and traditional medicinal uses. Sage tea has been used traditionally as a brain-enhancing tonic and extracts from sage have been reported to have both cognitive and memory enhancing effects. Brain-derived neurotrophic factor (BDNF) is an endogenous signaling molecule involved in cognition and memory function. In this study, activity-guided fractionation employing preparative reverse-phase high performance liquid chromatography (RP-HPLC) of culinary sage extracts led to the discovery of benzyl 6-O-β-D-apiofuranosyl-β-D-glucoside (B6AG) as a natural product that upregulates transcription of neurotrophic factors in C6 glioma cells. Purified B6AG showed a moderate dose response, with upregulation of BDNF and with EC at 6.46 μM. To better understand the natural variation in culinary sage, B6AG was quantitated in the leaves of several commercial varieties by liquid chromatography-mass spectrometry (LC-MS). The level of B6AG in dried culinary sage was found to range from 334 ± 14 to 698 ± 65 μg/g. This study provided a foundation for future investigations, including quantitative inquiries on the distribution of B6AG within the different plant organs, explorations in optimizing post-harvest practices, and aid in the development of sage varieties with elevated levels of B6AG.
食用鼠尾草（L.）是唇形科（Lamiaceae）中常见的香料植物，以其独特的烹饪和传统药用用途而闻名。鼠尾草茶传统上被用作增强大脑的滋补品，并且已经报道从鼠尾草中提取的物质具有认知和增强记忆力的作用。脑源性神经营养因子（BDNF）是一种内源性信号分子，参与认知和记忆功能。在这项研究中，采用活性导向的分离方法，通过制备型反相高效液相色谱（RP-HPLC）对食用鼠尾草提取物进行分离，发现了苯甲基 6-O-β-D-阿戊呋喃糖基-β-D-葡萄糖苷（B6AG）作为一种天然产物，可上调 C6 神经胶质瘤细胞中神经营养因子的转录。纯化的 B6AG 表现出适度的剂量反应，可上调 BDNF，EC 值为 6.46 μM。为了更好地了解商业品种食用鼠尾草的自然变异，通过液相色谱-质谱（LC-MS）对几种商业品种的叶片中的 B6AG 进行了定量分析。发现干食用鼠尾草中的 B6AG 含量范围为 334±14 至 698±65 μg/g。这项研究为未来的研究提供了基础，包括对 B6AG 在不同植物器官中的分布进行定量研究、探索优化收获后实践的方法以及开发 B6AG 含量更高的鼠尾草品种。
