北美和挪威慢性消耗病朊病毒表现出不同的种间传播潜力和人畜共患病风险。
North American and Norwegian Chronic Wasting Disease Prions Exhibit Different Potential for Interspecies Transmission and Zoonotic Risk.
机构信息
Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas McGovern Medical School at Houston, Houston, Texas, USA.
Prion Research Center, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
出版信息
J Infect Dis. 2022 Feb 1;225(3):542-551. doi: 10.1093/infdis/jiab385.
BACKGROUND
Chronic wasting disease (CWD) is a rapidly spreading prion disorder affecting various species of wild and captive cervids. The risk that CWD poses to cohabiting animals or more importantly to humans is largely unknown.
METHODS
In this study, we investigated differences in the capacity of CWD isolates obtained from 6 different cervid species to induce prion conversion in vitro by protein misfolding cyclic amplification. We define and quantify spillover and zoonotic potential indices as the efficiency by which CWD prions sustain prion generation in vitro at expenses of normal prion proteins from various mammals and human, respectively.
RESULTS
Our data suggest that reindeer and red deer from Norway could be the most transmissible CWD prions to other mammals, whereas North American CWD prions were more prone to generate human prions in vitro.
CONCLUSIONS
Our results suggest that Norway and North American CWD prions correspond to different strains with distinct spillover and zoonotic potentials.
背景
慢性消耗病（CWD）是一种快速传播的朊病毒疾病，影响各种野生和圈养的鹿科动物。CWD 对同居动物（或更重要的是对人类）构成的风险在很大程度上尚不清楚。
方法
在这项研究中，我们通过蛋白错误折叠循环扩增，研究了来自 6 种不同鹿种的 CWD 分离株在体外诱导朊病毒转化的能力差异。我们定义并量化了溢出和人畜共患病潜力指数，分别表示 CWD 朊病毒在体外以牺牲来自各种哺乳动物和人类的正常朊病毒蛋白的效率来维持朊病毒生成的效率。
结果
我们的数据表明，来自挪威的驯鹿和红鹿可能是朊病毒传播能力最强的 CWD 朊病毒，而北美的 CWD 朊病毒则更容易在体外生成人类朊病毒。
结论
我们的结果表明，挪威和北美的 CWD 朊病毒对应于具有不同溢出和人畜共患病潜力的不同株。
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