Sánchez-Tito Marco, Tay Lidia-Yileng
Facultad de Estomatología, Universidad Peruana Cayetano Heredia, Lima, Peru.
Facultad de Ciencias de la Salud, Universidad Privada de Tacna, Tacna, Peru.
J Clin Exp Dent. 2021 Jul 1;13(7):e685-e691. doi: 10.4317/jced.58330. eCollection 2021 Jul.
To evaluate the antibacterial property of a modified orthodontic resin with different concentrations of silver-nanoparticles (AgNPs), and quantify its preventive effect on the formation of white spot lesions (WSLs).
An orthodontic resin (Transbond XT) was modified with four concentrations of AgNPs (1%, 0.5%, 0.1%, and 0.05%), the orthodontic resin without AgNPs was used as control. Polymerized resin discs (n=80) were submitted to Agar diffusion test on Petri dishes inoculated with and . In addition, resin discs of each group (n=40) were placed in 96-well plates with bacterial suspensions to evaluate the colony-forming-units (CFU). For the WSLs prevention test, brackets were bonded with the experimental orthodontic resins on 45 premolars (n=5), and were subjected to a microbiological caries induction method for 9 days. Photographs were taken before and after the test, and the images were evaluated with the Image J software to calculate the area of WSLs. The data were analyzed using ANOVA and Tukey-HSD test, Student´s t-test and Kruskal-Wallis test (α=0.05).
The 0.5% and 1% AgNPs modified resin inhibit the growth of and . All the modified resins showed significantly less CFU, when compared to the control (<0.05). 1% AgNPs resin promote the higher prevention of WSLs formation. There was no significant difference between the control group and the 0.1% and 0.05% groups.
0.5% and 1% of AgNPs modified orthodontic resin exhibit an important antibacterial activity against and , and prevent the formation of WSLs. White spot lesions, antibacterial, orthodontics, adhesive, Silver-nanoparticles.
评估不同浓度银纳米颗粒（AgNPs）改性正畸树脂的抗菌性能，并量化其对白斑病变（WSLs）形成的预防效果。
用四种浓度的AgNPs（1%、0.5%、0.1%和0.05%）对正畸树脂（Transbond XT）进行改性，未添加AgNPs的正畸树脂作为对照。将聚合后的树脂圆盘（n = 80）置于接种了[具体细菌名称1]和[具体细菌名称2]的培养皿上进行琼脂扩散试验。此外，将每组的树脂圆盘（n = 40）置于含有细菌悬液的96孔板中，以评估菌落形成单位（CFU）。对于WSLs预防试验，将正畸实验树脂粘结在45颗前磨牙（n = 5）上，并采用微生物致龋诱导方法处理9天。在试验前后拍照，并用Image J软件评估图像，以计算WSLs的面积。数据采用方差分析和Tukey-HSD检验、Student's t检验和Kruskal-Wallis检验（α = 0.05）进行分析。
0.5%和1% AgNPs改性树脂抑制了[具体细菌名称1]和[具体细菌名称2]的生长。与对照组相比，所有改性树脂的CFU均显著减少（<0.05）。1% AgNPs树脂对WSLs形成的预防效果更佳。对照组与0.1%和0.05%组之间无显著差异。
0.5%和1%的AgNPs改性正畸树脂对[具体细菌名称1]和[具体细菌名称2]具有重要的抗菌活性，并能预防WSLs的形成。白斑病变、抗菌、正畸学、粘结剂、银纳米颗粒。
