Park R, Leach W J, Arieff A I
Am J Physiol. 1979 Mar;236(3):F240-5. doi: 10.1152/ajprenal.1979.236.3.F240.
An in vivo method is presented for the determination of liver intracellular pH (pHi) using [14C]dimethadione (DMO) in dogs. This method differs from those previously published in that hepatic venous and portal venous blood pH were selected as the extracellular reference pH, and liver blood space corrections are applied to whole liver tissue [14C]DMO activity. Using these corrections, a normal liver pHi of 6.99 +/- 0.03 (SE) was obtained. During acute metabolic acidosis and alkalosis, as well as during acute respiratory acidosis and alkalosis, the liver pHi remained normal; metabolic acidosis was 7.04 +/- 0.04; metabolic alkalosis was 6.92 +/- 0.08; respiratory acidosis was 6.98 +/- 0.04; and respiratory alkalosis was 7.00 +/- 0.10. None of these values was significantly different from normal (P greater than 0.05). Changes in intracellular bicarbonate and lactate appeared to account in part for the observed stability of the liver pHi despite acute manipulations resulting in a range of pH values between 7.09 and 7.63 in arterial blood.
本文介绍了一种在犬类中使用[14C]二甲双酮（DMO）测定肝脏细胞内pH值（pHi）的体内方法。该方法与先前发表的方法不同之处在于，选择肝静脉和门静脉血pH作为细胞外参考pH，并对全肝组织[14C]DMO活性进行肝血容量校正。通过这些校正，获得了正常肝脏pHi为6.99±0.03（标准误）。在急性代谢性酸中毒和碱中毒以及急性呼吸性酸中毒和碱中毒期间，肝脏pHi保持正常；代谢性酸中毒时为7.04±0.04；代谢性碱中毒时为6.92±0.08；呼吸性酸中毒时为6.98±0.04；呼吸性碱中毒时为7.00±0.10。这些值与正常相比均无显著差异（P>0.05）。尽管急性操作导致动脉血pH值在7.09至7.63之间变化，但细胞内碳酸氢盐和乳酸的变化似乎部分解释了观察到的肝脏pHi的稳定性。
