Department of Veterinary Sciences, University of Pisa, Italy.
Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Padova, Italy.
J Vet Diagn Invest. 2021 Nov;33(6):1069-1078. doi: 10.1177/10406387211035799. Epub 2021 Jul 31.
Numerous canine papillomaviruses (CPVs) have been identified (CPV1-23). CPV1, 2, and 6 have been associated with inverted papillomas (IPs). We retrieved 19 IPs from 3 histopathology archives, and evaluated and scored koilocytes, inclusion bodies, giant keratohyalin granules, cytoplasmic pallor, ballooning degeneration, and parakeratosis. IHC targeting major capsid proteins of PV was performed, and CPV genotyping was achieved by PCR testing. Tissue localization of CPV DNA and RNA was studied by chromogenic and RNAscope in situ hybridization (DNA-CISH, RNA-ISH, respectively). IPs were localized to the limbs (50%), trunk (30%), and head (20%), mainly as single nodules (16 of 19). In 15 of 19 cases, immunopositivity was detected within the nuclei in corneal and subcorneal epidermal layers. PCR revealed CPV1 in 11 IPs and CPV2 DNA in 3 IPs. Overall, 14 of 17 cases were positive by both DNA-CISH and RNA-ISH, in accord with PCR results. A histologic score >5 was always obtained in cases in which the viral etiology was demonstrated by IHC, DNA-CISH, and RNA-ISH. IHC and molecular approaches were useful to ascertain the viral etiology of IPs. Although IHC is the first choice for diagnostic purposes, ISH testing allows identification of PV type and the infection phase. RNA-ISH seems a promising tool to deepen our understanding of the pathogenesis of different PV types in animal species.
已鉴定出多种犬类乳头瘤病毒（CPV）（CPV1-23）。CPV1、2 和 6 与内翻性乳头状瘤（IP）有关。我们从 3 个组织病理学档案中检索到 19 个 IP，并对空泡化细胞、包涵体、巨大角蛋白颗粒、细胞质苍白、气球样变性和角化不良进行评估和评分。针对 PV 主要衣壳蛋白进行了 IHC，通过 PCR 检测实现了 CPV 基因分型。通过显色和 RNAscope 原位杂交（DNA-CISH、RNA-ISH）分别研究 CPV DNA 和 RNA 的组织定位。CPV DNA 和 RNA 的组织定位通过显色和 RNAscope 原位杂交（DNA-CISH、RNA-ISH）分别研究。IP 位于四肢（50%）、躯干（30%）和头部（20%），主要为单个结节（19 个中的 16 个）。在 19 例中的 15 例中，在角膜和表皮下表皮层的核内检测到免疫阳性。PCR 显示 11 个 IP 中存在 CPV1，3 个 IP 中存在 CPV2 DNA。总的来说，17 例中的 14 例通过 DNA-CISH 和 RNA-ISH 均为阳性，与 PCR 结果一致。在通过 IHC、DNA-CISH 和 RNA-ISH 证实病毒病因的病例中，组织评分>5 总是获得。IHC 和分子方法有助于确定 IP 的病毒病因。虽然 IHC 是诊断目的的首选方法，但 ISH 检测可识别 PV 类型和感染阶段。RNA-ISH 似乎是深入了解不同动物物种中不同 PV 类型发病机制的有前途的工具。
