Linder Keith E, Bizikova Petra, Luff Jennifer, Zhou Dan, Yuan Hang, Breuhaus Babetta, Nelson Elizabeth, Mackay Robert
Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, 1052 William Moore Drive, Raleigh, NC, 27607, USA.
Comparative Medicine Institute, North Carolina State University College of Veterinary Medicine, 1060 William Moore Drive, Raleigh, NC, 27607, USA.
Vet Dermatol. 2018 Feb;29(1):72-e30. doi: 10.1111/vde.12481. Epub 2017 Aug 22.
Currently, seven equine papillomaviruses (PV) are known and are associated with one of three different and distinct clinical presentations.
HYPOTHESIS/OBJECTIVES: To report the clinical, histopathological and immunohistochemical findings in horses with generalized papillomatosis associated with a novel equine PV, Equus caballus papillomavirus 8 (EcPV8).
Three client-owned quarter horses.
Case report, retrospective.
Dozens to thousands of papillomas involved the axilla, inguinal area and proximal limbs as well as the ventral and lateral neck, thorax and abdomen. Lesions were sometimes confluent in ventral areas. Fewer lesions were on the face, ears, distal limbs and genitalia. Plaque-type papillomas were small, 0.5 to 1.5 cm in diameter and hyperkeratotic. Histologically, plaque-type papillomas prominently involved follicular infundibula. Immunohistochemical findings demonstrated PV antigen in superficial keratinocyte nuclei. PCR using degenerate primers for the PV L1 gene and sequencing of amplicons revealed PV DNA sequences that were 98% identical for all three cases, but shared <70% identity to other PVs. Horses were otherwise healthy; serum immunoglobulin levels and peripheral blood lymphocyte phenotyping did not identify a known immunodeficiency syndrome. Lesions nearly completely resolved after 1.5 years in one horse and persisted for two years in another, despite intralesional human IFN-alpha treatment. The oldest horse was lost to follow-up.
A novel equine papillomavirus (EcPV8) is associated with a distinct, plaque-type, generalized papillomatosis. Papillomas persisted for months to years, with or without treatment.
目前，已知七种马乳头瘤病毒（PV），它们与三种不同且独特的临床表现之一相关。
假设/目的：报告与一种新型马PV，即马乳头瘤病毒8型（EcPV8）相关的全身性乳头瘤病马的临床、组织病理学和免疫组化结果。
三匹客户拥有的四分之一马。
病例报告，回顾性研究。
数十至数千个乳头瘤累及腋窝、腹股沟区和近端肢体，以及颈部腹侧和外侧、胸部和腹部。病变在腹侧区域有时融合。面部、耳部、远端肢体和生殖器上的病变较少。斑块型乳头瘤较小，直径为0.5至1.5厘米，且角化过度。组织学上，斑块型乳头瘤主要累及毛囊漏斗部。免疫组化结果显示PV抗原存在于表层角质形成细胞核中。使用针对PV L1基因的简并引物进行PCR及扩增子测序显示，所有三例的PV DNA序列有98%的同一性，但与其他PV的同一性小于70%。这些马在其他方面健康；血清免疫球蛋白水平和外周血淋巴细胞表型分析未发现已知的免疫缺陷综合征。尽管进行了病灶内注射人干扰素α治疗，但一匹马的病变在1.5年后几乎完全消退，另一匹马的病变持续了两年。最老的一匹马失访。
一种新型马乳头瘤病毒（EcPV8）与一种独特的、斑块型的全身性乳头瘤病相关。乳头瘤无论是否接受治疗，都持续数月至数年。
