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由矿化胶原蛋白和壳聚糖流延膜组成的双层膜，涂覆载有小檗碱的聚己内酯/聚乙烯吡咯烷酮电纺纳米纤维，可促进骨再生。

Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration.

作者信息

Zhang Yuhan, Wang Ting, Li Juan, Cui Xiaoming, Jiang Mingxia, Zhang Mogen, Wang Xiaoli, Zhang Weifen, Liu Zhijun

机构信息

Clinical College, Weifang Medical University, Weifang, China.

Shandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, China.

出版信息

Front Bioeng Biotechnol. 2021 Jul 19;9:684335. doi: 10.3389/fbioe.2021.684335. eCollection 2021.

DOI:10.3389/fbioe.2021.684335
PMID:34350160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8327095/
Abstract

Bone defects are difficult to repair and reconstruct as bone regeneration remains technically challenging, with exogenous factors required to accelerate this process. Biodegradable synthetic scaffolds are promising materials for stimulating bone tissue repair. In this study, we investigated whether a bilayer membrane that includes mineralized collagen (MC) and chitosan (CS) delivering berberine (BER)-a typical Chinese herbal monomer-could promote bone healing in a rat model. An MC/CS cast film was coated with polycaprolactone (PCL)/polyvinylpyrrolidone (PVP) electrospun nanofibers loaded with BER, yielding the BER@PCL/PVP-MC/CS bilayer membrane. The 3-dimensional structure had nanofibers of uniform diameter and showed good hydrophilicity; the bilayer membrane showed favorable mechanical properties. BER@PCL/PVP-MC/CS enhanced the proliferation and attachment of MC3T3-E1 cells and induced bone regeneration when implanted into a rat femoral bone defect. These findings provide evidence that BER@PCL/PVP-MC/CS has clinical potential for effective bone repair.

摘要

骨缺损难以修复和重建，因为骨再生在技术上仍然具有挑战性，需要外源性因素来加速这一过程。可生物降解的合成支架是刺激骨组织修复的有前途的材料。在本研究中，我们研究了一种包含矿化胶原（MC）和壳聚糖（CS）并递送黄连素（BER，一种典型的中草药单体）的双层膜是否能促进大鼠模型中的骨愈合。将MC/CS流延膜用负载有BER的聚己内酯（PCL）/聚乙烯吡咯烷酮（PVP）电纺纳米纤维进行包覆，得到BER@PCL/PVP-MC/CS双层膜。该三维结构具有直径均匀的纳米纤维且表现出良好的亲水性；双层膜表现出良好的力学性能。BER@PCL/PVP-MC/CS增强了MC3T3-E1细胞的增殖和附着，并在植入大鼠股骨骨缺损时诱导了骨再生。这些发现提供了证据，表明BER@PCL/PVP-MC/CS在有效骨修复方面具有临床潜力。

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