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利用高通量单像素压缩全息术对生物组织进行成像。

Imaging biological tissue with high-throughput single-pixel compressive holography.

机构信息

Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China.

Guangdong Provincial Key Labratory of Optoelectronic Information Processing Chips and Systems, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China.

出版信息

Nat Commun. 2021 Aug 5;12(1):4712. doi: 10.1038/s41467-021-24990-0.

DOI:10.1038/s41467-021-24990-0
PMID:34354073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8342474/
Abstract

Single-pixel holography (SPH) is capable of generating holographic images with rich spatial information by employing only a single-pixel detector. Thanks to the relatively low dark-noise production, high sensitivity, large bandwidth, and cheap price of single-pixel detectors in comparison to pixel-array detectors, SPH is becoming an attractive imaging modality at wavelengths where pixel-array detectors are not available or prohibitively expensive. In this work, we develop a high-throughput single-pixel compressive holography with a space-bandwidth-time product (SBP-T) of 41,667 pixels/s, realized by enabling phase stepping naturally in time and abandoning the need for phase-encoded illumination. This holographic system is scalable to provide either a large field of view (~83 mm) or a high resolution (5.80 μm × 4.31 μm). In particular, high-resolution holographic images of biological tissues are presented, exhibiting rich contrast in both amplitude and phase. This work is an important step towards multi-spectrum imaging using a single-pixel detector in biophotonics.

摘要

单像素全息术 (SPH) 通过仅使用单个像素探测器就能生成具有丰富空间信息的全息图像。由于单像素探测器的暗噪声产生率相对较低、灵敏度高、带宽大且价格便宜,与像素阵列探测器相比,SPH 在像素阵列探测器不可用或过于昂贵的波长下成为一种极具吸引力的成像方式。在这项工作中,我们通过自然地在时间上实现相位步进并放弃对相编码照明的需求,开发了一种具有 41,667 像素/秒的高吞吐量单像素压缩全息术,实现了空间带宽时间乘积 (SBP-T)。该全息系统具有可扩展性,可提供大视野(~83mm)或高分辨率(5.80μm×4.31μm)。特别地,展示了生物组织的高分辨率全息图像,在幅度和相位上都具有丰富的对比度。这项工作是朝着在生物光子学中使用单像素探测器进行多光谱成像迈出的重要一步。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/b41b456f4456/41467_2021_24990_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/647437366d30/41467_2021_24990_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/18be0ab0550f/41467_2021_24990_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/fb45dc24b3b8/41467_2021_24990_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/5d7ef617d164/41467_2021_24990_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/e99be2eeca80/41467_2021_24990_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/2ad250af7f28/41467_2021_24990_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/b41b456f4456/41467_2021_24990_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/647437366d30/41467_2021_24990_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/18be0ab0550f/41467_2021_24990_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/fb45dc24b3b8/41467_2021_24990_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/5d7ef617d164/41467_2021_24990_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/e99be2eeca80/41467_2021_24990_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/2ad250af7f28/41467_2021_24990_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6d/8342474/b41b456f4456/41467_2021_24990_Fig7_HTML.jpg

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