Santos Tárcio S, Silva Tarcisio M, Cardoso Juliana C, Albuquerque-Júnior Ricardo L C de, Zielinska Aleksandra, Souto Eliana B, Severino Patrícia, Mendonça Marcelo da Costa
University of Tiradentes (Unit), Av. Murilo Dantas, Aracaju 49010-390, Brazil.
Nanomedicine and Nanotechnology Laboratory (LNMed), Institute of Technology and Research (ITP), Av. Murilo Dantas, Aracaju 49010-390, Brazil.
Antibiotics (Basel). 2021 Jul 13;10(7):852. doi: 10.3390/antibiotics10070852.
Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.
由于其多功能性，银纳米颗粒在生物医学和农业食品领域得到广泛应用。由于其可行性和高生物相容性，利用生物方法合成银纳米颗粒的情况已大幅增加。一般来说，微生物已被广泛探索用于生产多种应用的银纳米颗粒。这项工作的目的是评估与其他丝状真菌相比，昆虫病原真菌用于生物合成银纳米颗粒的情况，以及使用这些纳米颗粒作为抗菌剂和控制害虫的可能性。此外，还讨论了常用于评估这些材料毒性的体外方法。已知几种丝状真菌有形成银纳米颗粒的能力，但关于昆虫病原真菌产生这些材料的潜力的研究很少。银纳米颗粒毒性的研究通常在体外通过细胞毒性/遗传毒性分析进行，分别使用成熟的方法，如MTT和彗星试验。利用昆虫病原真菌获得的银纳米颗粒防治昆虫主要集中在传播疾病给人类的蚊子上，在死亡率估计方面取得了令人满意的结果。昆虫病原真菌可用于合成银纳米颗粒，潜在用于昆虫控制，但需要扩大毒性研究，以便使其也能用于农业害虫控制。
