Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, China.
School of Basic Medical Sciences, Baicheng Medical College, Baicheng, 137000, China.
Sci Rep. 2021 May 14;11(1):10356. doi: 10.1038/s41598-021-89854-5.
Due to the increasing demand for eco-friendly, cost-effective and safe technologies, biosynthetic metal nanoparticles have attracted worldwide attention. In this study, silver nanoparticles (AgNPs) were extracellularly biosynthesized using the culture supernatants of Aspergillus sydowii. During synthesis, color change was preliminarily judge of the generation of AgNPs, and the UV absorption peak at 420 nm further confirms the production of AgNPs. Transmission electron microscopy and X-ray diffraction were also used to identify the AgNPs. The results shows that AgNPs has crystalline cubic feature and is a polydisperse spherical particle with size between 1 and 24 nm. Three main synthesis factors (temperature, pH and substrate concentration) were optimized, the best synthesis conditions were as follows 50 °C, 8.0 and 1.5 mM. In the biological application of AgNPs, it shows effective antifungal activity against many clinical pathogenic fungi and antiproliferative activity to HeLa cells and MCF-7 cells in vitro. Our research finds a new path to biosynthesis of AgNPs in an eco-friendly manner, and bring opportunity for biomedical applications in clinic.
由于对环保、经济高效和安全技术的需求不断增加，生物合成金属纳米粒子引起了全世界的关注。在本研究中，使用泡盛曲霉（Aspergillus sydowii）的培养上清液体外生物合成了银纳米粒子（AgNPs）。在合成过程中，颜色变化初步判断 AgNPs 的生成，而在 420nm 处的紫外吸收峰进一步证实了 AgNPs 的生成。透射电子显微镜和 X 射线衍射也用于鉴定 AgNPs。结果表明，AgNPs 具有立方晶态特征，是一种多分散的球形颗粒，粒径在 1 至 24nm 之间。优化了三个主要的合成因素（温度、pH 值和基质浓度），最佳合成条件为 50°C、8.0 和 1.5mM。在 AgNPs 的生物应用中，它对许多临床致病性真菌表现出有效的抗真菌活性，对 HeLa 细胞和 MCF-7 细胞具有体外增殖活性。我们的研究为环保方式生物合成 AgNPs 找到了一条新途径，并为临床生物医学应用带来了机会。
