Bernad-Roche María, Bellés Andrea, Grasa Laura, Casanova-Higes Alejandro, Mainar-Jaime Raúl Carlos
Departamento de Patología Animal, Facultad de Veterinaria, Instituto Agroalimentario de Aragón-IA2, Universidad de Zaragoza-CITA, 50013 Zaragoza, Spain.
Departamento de Farmacología y Fisiología, Facultad de Veterinaria, Instituto Agroalimentario de Aragón-IA2, Universidad de Zaragoza-CITA, 50013 Zaragoza, Spain.
Animals (Basel). 2021 Jul 19;11(7):2137. doi: 10.3390/ani11072137.
The study assessed changes in the gut microbiota of pigs after dietary supplementation with protected sodium butyrate (PSB) during the growing-fattening period (≈90 days). One gram of colon content from 18 pigs (9 from the treatment group -TG- and 9 from the control group -CG-) was collected. Bacterial DNA was extracted and 16S rRNA high-throughput amplicon sequencing used to assess microbiota changes between groups. The groups shared 75.4% of the 4697 operational taxonomic units identified. No differences in alpha diversity were found, but significant differences for some specific taxa were detected between groups. The low-represented phylum , which is associated with the production of carotenoids with antioxidant, anti-apoptotic, and anti-inflammatory properties, was increased in the TG ( = 0.032). , , , , and were increased in the TG. Members of these families have the ability to ferment complex dietary polysaccharides and produce larger amounts of short chain fatty acids. Regarding species, only was increased in the TG ( = 0.048). is well-known as probiotic in humans, but it has also been associated with overall positive gut effects (increased villus height, improved body weight, reduction of diarrhea, etc.) in weanling pigs. Although the use of PSB did not modify the overall richness of microbiota composition of these slaughter pigs, it may have increased specific taxa associated with better gut health parameters.
本研究评估了生长育肥期（约90天）日粮添加保护型丁酸钠（PSB）后猪肠道微生物群的变化。采集了18头猪（9头来自处理组-TG-，9头来自对照组-CG-）的1克结肠内容物。提取细菌DNA，并使用16S rRNA高通量扩增子测序来评估组间微生物群的变化。在鉴定出的4697个操作分类单元中，两组共有75.4%。未发现α多样性存在差异，但检测到两组之间某些特定分类群存在显著差异。在TG组中，与具有抗氧化、抗凋亡和抗炎特性的类胡萝卜素产生相关的低丰度门有所增加（P = 0.032）。TG组中，毛螺菌科、瘤胃菌科、真杆菌科、双歧杆菌科和肠球菌科有所增加。这些科的成员有能力发酵复杂的膳食多糖并产生大量短链脂肪酸。在物种方面，TG组中只有嗜黏蛋白阿克曼菌增加（P = 0.048）。嗜黏蛋白阿克曼菌在人类中是众所周知的益生菌，但它也与断奶仔猪的整体肠道积极影响（绒毛高度增加、体重改善、腹泻减少等）有关。虽然使用PSB并没有改变这些屠宰猪微生物群组成的总体丰富度，但它可能增加了与更好的肠道健康参数相关的特定分类群。
