Chai Liang, Zhang Jinfang, Li Haojie, Cui Cheng, Jiang Jun, Zheng Benchuan, Wu Lintao, Jiang Liangcai
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
School of Biological Sciences, Guizhou Education University, Guiyang, China.
Front Genet. 2021 Jul 23;12:678804. doi: 10.3389/fgene.2021.678804. eCollection 2021.
In higher plants, the structure of a flower is precisely controlled by a series of genes. An aberrance flower results in abnormal fruit morphology. Previously, we reported multi-silique rapeseed () line zws-ms. We identified two associated regions and investigated differentially expressed genes (DEGs); thus, some candidate genes underlying the multi-silique phenotype in warm area Xindu were selected. However, this phenotype was switched off by lower temperature, and the responsive genes, known as thermomorphogenesis-related genes, remained elusive. So, based on that, in this study, we further investigated the transcriptome data from buds of zws-ms and its near-isogenic line zws-217 grown in colder area Ma'erkang, where both lines showed normal siliques only, and the DEGs between them analyzed. We compared the 129 DEGs from Xindu to the 117 ones from Ma'erkang and found that 33 of them represented the same or similar expression trends, whereas the other 96 DEGs showed different expression trends, which were defined as environment-specific. Furthermore, we combined this with the gene annotations and ortholog information and then selected BnaA09g45320D (chaperonin gene -homologous) and BnaC08g41780D [Seryl-tRNA synthetase gene ()-homologous] the possible thermomorphogenesis-related genes, which probably switched off the multi-silique under lower temperature. This study paves a way to a new perspective into flower/fruit development in plants.
在高等植物中，花的结构由一系列基因精确控制。花的异常会导致果实形态异常。此前，我们报道了多角果油菜（）品系zws-ms。我们鉴定了两个相关区域并研究了差异表达基因（DEG）；因此，选择了一些位于温暖地区新都的多角果表型潜在的候选基因。然而，这种表型在较低温度下会消失，而被称为热形态建成相关基因的响应基因仍然未知。因此，基于此，在本研究中，我们进一步研究了zws-ms及其近等基因系zws-217在较寒冷地区马尔康生长的芽的转录组数据，在那里两个品系仅表现出正常的角果，并分析了它们之间的DEG。我们将来自新都的129个DEG与来自马尔康的117个DEG进行比较，发现其中33个呈现相同或相似的表达趋势，而其他96个DEG表现出不同的表达趋势，这些被定义为环境特异性的。此外，我们将此与基因注释和直系同源信息相结合，然后选择了BnaA09g45320D（伴侣蛋白基因 -同源）和BnaC08g41780D [丝氨酰 -tRNA合成酶基因 （）-同源]作为可能的热形态建成相关基因，它们可能在较低温度下关闭了多角果表型。本研究为油菜植物花/果实发育的新视角铺平了道路。
