Laboratory of Microbiological Engineering and Application, Department of Biochemistry and Molecular and Cellular Biology, University of Mentouri Brothers Constantine 1, P.O. Box, 325 Ain El Bey Way, Constantine, 25017, Algeria.
Laboratory of Microbiological Engineering and Application, Department of Biochemistry and Molecular and Cellular Biology, University of Mentouri Brothers Constantine 1, Constantine 25017, Algeria.
Int J Med Mushrooms. 2021;23(6):79-88. doi: 10.1615/IntJMedMushrooms.2021038424.
Mushrooms can be employed as functional foods or medicines for the prevention and treatment of several chronic diseases, since they are rich sources of bioactive metabolites. Nonetheless, the therapeutic value of Algerian mushrooms remains largely unexplored. To date, there are no published studies concerning the different medicinal properties of endemic Ganoderma lucidum in Algeria. This study aimed to compare the antioxidant potential of an endemic specimen collected in the Lake Tonga Forest of El Kala National Park in Northeast Algeria with findings from other countries. To this end, phenolic and flavonoid contents were determined using three solvents of increasing polarity (chloroform, ethyl acetate, and butanol). The mushroom specimen was identified as G. lucidum based on strong molecular evidence using internal transcribed spacer, large subunit, and small subunit sequences. Our study showed that ethyl acetate extract contained higher total phenolic content (171.1 ± 1.06 μg gallic acid equivalents/mg and 102.5 ± 0.69 μg pyrocatechol equivalents/mg extract) and total flavonoid content (25.48 ± 0.13 μg quercetin equivalents/mg and 40.45 ± 0.83 μg rutin equivalents/mg extract) than the other mushroom extracts (chloroform and butanolic). The ethyl acetate extract also showed good antioxidant activity in DPPH (28.51 ± 0.24 μg/mL), ABTS·+ (10.06 ± 0.13 μg/mL), galvinoxyl radical (15.46 ± 0.48 μg/mL), reducing power (22.74 ± 0.30 μg/mL), cupric reducing antioxidant capacity (21.36 ± 0.04 μg/mL), and phenanthroline (12.87 ± 0.26 μg/mL) assays. These results suggest that G. lucidum could be used as a source of strong natural antioxidants for the food and pharmaceutical industries.
蘑菇可以作为功能性食品或药物，用于预防和治疗多种慢性疾病，因为它们是生物活性代谢物的丰富来源。然而，阿尔及利亚蘑菇的治疗价值在很大程度上仍未得到探索。迄今为止，尚无关于阿尔及利亚特有灵芝的不同药用特性的已发表研究。本研究旨在比较在阿尔及利亚东北部 El Kala 国家公园 Tonga 湖森林中采集的特有标本与其他国家的研究结果的抗氧化潜力。为此，使用三种极性递增的溶剂（氯仿、乙酸乙酯和正丁醇）来确定酚类和类黄酮的含量。根据内部转录间隔区、大亚基和小亚基序列的强烈分子证据，该蘑菇标本被鉴定为灵芝。我们的研究表明，乙酸乙酯提取物含有较高的总酚含量（171.1 ± 1.06 μg 没食子酸当量/毫克和 102.5 ± 0.69 μg 邻苯二酚当量/毫克提取物）和总类黄酮含量（25.48 ± 0.13 μg 槲皮素当量/毫克和 40.45 ± 0.83 μg 芦丁当量/毫克提取物）比其他蘑菇提取物（氯仿和正丁醇）高。乙酸乙酯提取物在 DPPH（28.51 ± 0.24 μg/mL）、ABTS·+（10.06 ± 0.13 μg/mL）、戊二醛自由基（15.46 ± 0.48 μg/mL）、还原能力（22.74 ± 0.30 μg/mL）、铜还原抗氧化能力（21.36 ± 0.04 μg/mL）和邻菲啰啉（12.87 ± 0.26 μg/mL）测定中也表现出良好的抗氧化活性。这些结果表明，灵芝可作为食品和制药工业中强天然抗氧化剂的来源。
